1djm: Difference between revisions
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New page: left|200px<br /><applet load="1djm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1djm" /> '''SOLUTION STRUCTURE OF BEF3-ACTIVATED CHEY FR... |
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[[Image:1djm.jpg|left|200px]]<br /><applet load="1djm" size=" | [[Image:1djm.jpg|left|200px]]<br /><applet load="1djm" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1djm" /> | caption="1djm" /> | ||
'''SOLUTION STRUCTURE OF BEF3-ACTIVATED CHEY FROM ESCHERICHIA COLI'''<br /> | '''SOLUTION STRUCTURE OF BEF3-ACTIVATED CHEY FROM ESCHERICHIA COLI'''<br /> | ||
==Overview== | ==Overview== | ||
The CheY protein is the response regulator in bacterial chemotaxis. | The CheY protein is the response regulator in bacterial chemotaxis. Phosphorylation of a conserved aspartyl residue induces structural changes that convert the protein from an inactive to an active state. The short half-life of the aspartyl-phosphate has precluded detailed structural analysis of the active protein. Persistent activation of Escherichia coli CheY was achieved by complexation with beryllofluoride (BeF(3)(-)) and the structure determined by NMR spectroscopy to a backbone r.m.s.d. of 0.58(+/-0.08) A. Formation of a hydrogen bond between the Thr87 OH group and an active site acceptor, presumably Asp57.BeF(3)(-), stabilizes a coupled rearrangement of highly conserved residues, Thr87 and Tyr106, along with displacement of beta4 and H4, to yield the active state. The coupled rearrangement may be a more general mechanism for activation of receiver domains. | ||
==About this Structure== | ==About this Structure== | ||
1DJM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | 1DJM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJM OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Cho, H | [[Category: Cho, H S.]] | ||
[[Category: Kustu, S.]] | [[Category: Kustu, S.]] | ||
[[Category: Lee, S | [[Category: Lee, S Y.]] | ||
[[Category: Pan, X.]] | [[Category: Pan, X.]] | ||
[[Category: Parkinson, J | [[Category: Parkinson, J S.]] | ||
[[Category: Pelton, J | [[Category: Pelton, J G.]] | ||
[[Category: Wemmer, D | [[Category: Wemmer, D E.]] | ||
[[Category: Yan, D.]] | [[Category: Yan, D.]] | ||
[[Category: befx]] | [[Category: befx]] | ||
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[[Category: two-component]] | [[Category: two-component]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:17:13 2008'' | ||
