1ec6: Difference between revisions

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[[Image:1ec6.gif|left|200px]]<br />

[[Image:1ec6.gif|left|200px]]<br /><applet load="1ec6" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1ec6" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1ec6, resolution 2.40Å" />

'''CRYSTAL STRUCTURE OF NOVA-2 KH3 K-HOMOLOGY RNA-BINDING DOMAIN BOUND TO 20-MER RNA HAIRPIN'''<br />

==Overview==

The structure of a Nova protein K homology (KH) domain recognizing, single-stranded RNA has been determined at 2.4 A resolution. Mammalian, Nova antigens (1 and 2) constitute an important family of regulators of, RNA metabolism in neurons, first identified using sera from cancer, patients with the autoimmune disorder paraneoplastic opsoclonus-myoclonus, ataxia (POMA). The structure of the third KH domain (KH3) of Nova-2 bound, to a stem loop RNA resembles a molecular vise, with 5'-Ura-Cyt-Ade-Cyt-3', pinioned between an invariant Gly-X-X-Gly motif and the variable loop., Tetranucleotide recognition is supported by an aliphatic alpha helix/beta, sheet RNA-binding platform, which mimics 5'-Ura-Gua-3' by making, Watson-Crick-like hydrogen bonds with 5'-Cyt-Ade-3'. Sequence conservation, suggests that fragile X mental retardation results from perturbation of, RNA binding by the FMR1 protein.

The structure of a Nova protein K homology (KH) domain recognizing single-stranded RNA has been determined at 2.4 A resolution. Mammalian Nova antigens (1 and 2) constitute an important family of regulators of RNA metabolism in neurons, first identified using sera from cancer patients with the autoimmune disorder paraneoplastic opsoclonus-myoclonus ataxia (POMA). The structure of the third KH domain (KH3) of Nova-2 bound to a stem loop RNA resembles a molecular vise, with 5'-Ura-Cyt-Ade-Cyt-3' pinioned between an invariant Gly-X-X-Gly motif and the variable loop. Tetranucleotide recognition is supported by an aliphatic alpha helix/beta sheet RNA-binding platform, which mimics 5'-Ura-Gua-3' by making Watson-Crick-like hydrogen bonds with 5'-Cyt-Ade-3'. Sequence conservation suggests that fragile X mental retardation results from perturbation of RNA binding by the FMR1 protein.

==About this Structure==

1EC6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1EC6 OCA].

1EC6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EC6 OCA].

==Reference==

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[[Category: Chen, H.]]

[[Category: Edo, C.]]

[[Category: Jensen, K.B.]]

[[Category: Jensen, K B.]]

[[Category: Lewis, H.A.]]

[[Category: Lewis, H A.]]

[[Category: Musunuru, K.]]

[[Category: alpha-beta fold]]

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[[Category: rna-binding motif]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov~~ 12 16:42:~~03 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:26:10 2008''

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-[[Image:1ec6.gif|left|200px]]<br />
+[[Image:1ec6.gif|left|200px]]<br /><applet load="1ec6" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1ec6" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1ec6, resolution 2.40&Aring;" />
 '''CRYSTAL STRUCTURE OF NOVA-2 KH3 K-HOMOLOGY RNA-BINDING DOMAIN BOUND TO 20-MER RNA HAIRPIN'''<br />
 ==Overview==
-The structure of a Nova protein K homology (KH) domain recognizing, single-stranded RNA has been determined at 2.4 A resolution. Mammalian, Nova antigens (1 and 2) constitute an important family of regulators of, RNA metabolism in neurons, first identified using sera from cancer, patients with the autoimmune disorder paraneoplastic opsoclonus-myoclonus, ataxia (POMA). The structure of the third KH domain (KH3) of Nova-2 bound, to a stem loop RNA resembles a molecular vise, with 5'-Ura-Cyt-Ade-Cyt-3', pinioned between an invariant Gly-X-X-Gly motif and the variable loop., Tetranucleotide recognition is supported by an aliphatic alpha helix/beta, sheet RNA-binding platform, which mimics 5'-Ura-Gua-3' by making, Watson-Crick-like hydrogen bonds with 5'-Cyt-Ade-3'. Sequence conservation, suggests that fragile X mental retardation results from perturbation of, RNA binding by the FMR1 protein.
+The structure of a Nova protein K homology (KH) domain recognizing single-stranded RNA has been determined at 2.4 A resolution. Mammalian Nova antigens (1 and 2) constitute an important family of regulators of RNA metabolism in neurons, first identified using sera from cancer patients with the autoimmune disorder paraneoplastic opsoclonus-myoclonus ataxia (POMA). The structure of the third KH domain (KH3) of Nova-2 bound to a stem loop RNA resembles a molecular vise, with 5'-Ura-Cyt-Ade-Cyt-3' pinioned between an invariant Gly-X-X-Gly motif and the variable loop. Tetranucleotide recognition is supported by an aliphatic alpha helix/beta sheet RNA-binding platform, which mimics 5'-Ura-Gua-3' by making Watson-Crick-like hydrogen bonds with 5'-Cyt-Ade-3'. Sequence conservation suggests that fragile X mental retardation results from perturbation of RNA binding by the FMR1 protein.
 ==About this Structure==
-EC6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EC6 OCA].
+EC6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EC6 OCA].
 ==Reference==
@@ Line 16: / Line 15: @@
 [[Category: Chen, H.]]
 [[Category: Edo, C.]]
-[[Category: Jensen, K.B.]]
+[[Category: Jensen, K B.]]
-[[Category: Lewis, H.A.]]
+[[Category: Lewis, H A.]]
 [[Category: Musunuru, K.]]
 [[Category: alpha-beta fold]]
@@ Line 24: / Line 23: @@
 [[Category: rna-binding motif]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:42:03 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:26:10 2008''