1el4: Difference between revisions
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New page: left|200px<br /><applet load="1el4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1el4, resolution 1.73Å" /> '''STRUCTURE OF THE CAL... |
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[[Image:1el4.gif|left|200px]]<br /><applet load="1el4" size=" | [[Image:1el4.gif|left|200px]]<br /><applet load="1el4" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1el4, resolution 1.73Å" /> | caption="1el4, resolution 1.73Å" /> | ||
'''STRUCTURE OF THE CALCIUM-REGULATED PHOTOPROTEIN OBELIN DETERMINED BY SULFUR SAS'''<br /> | '''STRUCTURE OF THE CALCIUM-REGULATED PHOTOPROTEIN OBELIN DETERMINED BY SULFUR SAS'''<br /> | ||
==Overview== | ==Overview== | ||
The crystal structure of the photoprotein obelin (22.2 kDa) from Obelia | The crystal structure of the photoprotein obelin (22.2 kDa) from Obelia longissima has been determined and refined to 1.7 A resolution. Contrary to the prediction of a peroxide, the noncovalently bound substrate, coelenterazine, has only a single oxygen atom bound at the C2-position. The protein-coelenterazine 2-oxy complex observed in the crystals is photo-active because, in the presence of calcium ion, bioluminescence emission within the crystal is observed. This structure represents only the second de novo protein structure determined using the anomalous scattering signal of the sulfur substructure in the crystal. The method used here is theoretically different from that used for crambin in 1981 (4.72 kDa) and represents a significant advancement in protein crystal structure determination. | ||
==About this Structure== | ==About this Structure== | ||
1EL4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Obelia_longissima Obelia longissima] with CL and CTZ as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1EL4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Obelia_longissima Obelia longissima] with <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=CTZ:'>CTZ</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EL4 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Lee, J.]] | [[Category: Lee, J.]] | ||
[[Category: Liu, Z | [[Category: Liu, Z J.]] | ||
[[Category: Rose, J.]] | [[Category: Rose, J.]] | ||
[[Category: Vysotski, E | [[Category: Vysotski, E S.]] | ||
[[Category: Wang, B | [[Category: Wang, B C.]] | ||
[[Category: CL]] | [[Category: CL]] | ||
[[Category: CTZ]] | [[Category: CTZ]] | ||
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[[Category: sulfur anomalous scattering]] | [[Category: sulfur anomalous scattering]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:28:53 2008'' | ||
