1f1z: Difference between revisions

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[[Image:1f1z.gif|left|200px]]<br /><applet load="1f1z" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1f1z.gif|left|200px]]<br /><applet load="1f1z" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1f1z, resolution 2.40Å" />

'''TNSA, a catalytic component of the TN7 transposition system'''<br />

==Overview==

Transposition requires a coordinated series of DNA breakage and joining, reactions. The Tn7 transposase contains two proteins: TnsA, which carries, out DNA breakage at the 5' ends of the transposon, and TnsB, which carries, out breakage and joining at the 3' ends of the transposon. TnsB is a, member of the retroviral integrase superfamily whose hallmark is a, conserved DDE motif. We report here the structure of TnsA at 2.4 A, resolution. Surprisingly, the TnsA fold is that of a type II restriction, endonuclease. Thus, Tn7 transposition involves a collaboration between, polypeptides, one containing a DDE motif and one that does not. This, result indicates that the range of biological processes that utilize, restriction enzyme-like folds also includes DNA transposition.

Transposition requires a coordinated series of DNA breakage and joining reactions. The Tn7 transposase contains two proteins: TnsA, which carries out DNA breakage at the 5' ends of the transposon, and TnsB, which carries out breakage and joining at the 3' ends of the transposon. TnsB is a member of the retroviral integrase superfamily whose hallmark is a conserved DDE motif. We report here the structure of TnsA at 2.4 A resolution. Surprisingly, the TnsA fold is that of a type II restriction endonuclease. Thus, Tn7 transposition involves a collaboration between polypeptides, one containing a DDE motif and one that does not. This result indicates that the range of biological processes that utilize restriction enzyme-like folds also includes DNA transposition.

==About this Structure==

1F1Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1F1Z OCA].

1F1Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F1Z OCA].

==Reference==

Line 13:

[[Category: Escherichia coli]]

[[Category: Single protein]]

[[Category: Craig, N.L.]]

[[Category: Craig, N L.]]

[[Category: Dyda, F.]]

[[Category: Hickman, A.B.]]

[[Category: Hickman, A B.]]

[[Category: Li, Y.]]

[[Category: Mathew, S.V.]]

[[Category: Mathew, S V.]]

[[Category: May, E.W.]]

[[Category: May, E W.]]

[[Category: CL]]

[[Category: MG]]

[[Category: restriction endonuclease fold]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 14~~:31:~~54 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:33:55 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1f1z.gif|left|200px]]<br /><applet load="1f1z" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1f1z.gif|left|200px]]<br /><applet load="1f1z" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1f1z, resolution 2.40&Aring;" />
 '''TNSA, a catalytic component of the TN7 transposition system'''<br />
 ==Overview==
-Transposition requires a coordinated series of DNA breakage and joining, reactions. The Tn7 transposase contains two proteins: TnsA, which carries, out DNA breakage at the 5' ends of the transposon, and TnsB, which carries, out breakage and joining at the 3' ends of the transposon. TnsB is a, member of the retroviral integrase superfamily whose hallmark is a, conserved DDE motif. We report here the structure of TnsA at 2.4 A, resolution. Surprisingly, the TnsA fold is that of a type II restriction, endonuclease. Thus, Tn7 transposition involves a collaboration between, polypeptides, one containing a DDE motif and one that does not. This, result indicates that the range of biological processes that utilize, restriction enzyme-like folds also includes DNA transposition.
+Transposition requires a coordinated series of DNA breakage and joining reactions. The Tn7 transposase contains two proteins: TnsA, which carries out DNA breakage at the 5' ends of the transposon, and TnsB, which carries out breakage and joining at the 3' ends of the transposon. TnsB is a member of the retroviral integrase superfamily whose hallmark is a conserved DDE motif. We report here the structure of TnsA at 2.4 A resolution. Surprisingly, the TnsA fold is that of a type II restriction endonuclease. Thus, Tn7 transposition involves a collaboration between polypeptides, one containing a DDE motif and one that does not. This result indicates that the range of biological processes that utilize restriction enzyme-like folds also includes DNA transposition.
 ==About this Structure==
-F1Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F1Z OCA].
+F1Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F1Z OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
-[[Category: Craig, N.L.]]
+[[Category: Craig, N L.]]
 [[Category: Dyda, F.]]
-[[Category: Hickman, A.B.]]
+[[Category: Hickman, A B.]]
 [[Category: Li, Y.]]
-[[Category: Mathew, S.V.]]
+[[Category: Mathew, S V.]]
-[[Category: May, E.W.]]
+[[Category: May, E W.]]
 [[Category: CL]]
 [[Category: MG]]
 [[Category: restriction endonuclease fold]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:31:54 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:33:55 2008''