1fdz: Difference between revisions
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New page: left|200px<br /><applet load="1fdz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fdz, resolution 2.6Å" /> '''N-ACETYLNEURAMINATE L... |
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[[Image:1fdz.gif|left|200px]]<br /><applet load="1fdz" size=" | [[Image:1fdz.gif|left|200px]]<br /><applet load="1fdz" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1fdz, resolution 2.6Å" /> | caption="1fdz, resolution 2.6Å" /> | ||
'''N-ACETYLNEURAMINATE LYASE IN COMPLEX WITH PYRUVATE VIA BOROHYDRIDE REDUCTION'''<br /> | '''N-ACETYLNEURAMINATE LYASE IN COMPLEX WITH PYRUVATE VIA BOROHYDRIDE REDUCTION'''<br /> | ||
==Overview== | ==Overview== | ||
We describe here a sub-family of enzymes related both structurally and | We describe here a sub-family of enzymes related both structurally and functionally to N-acetylneuraminate lyase. Two members of this family (N-acetylneuraminate lyase and dihydrodipicolinate synthase) have known three-dimensional structures and we now proceed to show their structural and functional relationship to two further proteins, trans-o-hydroxybenzylidenepyruvate hydratase-aldolase and D-4-deoxy-5-oxoglucarate dehydratase. These enzymes are all thought to involve intermediate Schiff-base formation with their respective substrates. In order to understand the nature of this intermediate, we have determined the three-dimensional structure of N-acetylneuraminate lyase in complex with hydroxypyruvate (a product analogue) and in complex with one of its products (pyruvate). From these structures we deduce the presence of a closely similar Schiff-base forming motif in all members of the N-acetylneuraminate lyase sub-family. A fifth protein, MosA, is also confirmed to be a member of the sub-family although the involvement of an intermediate Schiff-base in its proposed reaction is unclear. | ||
==About this Structure== | ==About this Structure== | ||
1FDZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with PYR as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/N-acetylneuraminate_lyase N-acetylneuraminate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.3 4.1.3.3] Full crystallographic information is available from [http:// | 1FDZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=PYR:'>PYR</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/N-acetylneuraminate_lyase N-acetylneuraminate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.3 4.1.3.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FDZ OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: N-acetylneuraminate lyase]] | [[Category: N-acetylneuraminate lyase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Barbosa, J | [[Category: Barbosa, J A.R G.]] | ||
[[Category: Hall, N | [[Category: Hall, N E.]] | ||
[[Category: Lawrence, M | [[Category: Lawrence, M C.]] | ||
[[Category: Marcuccio, S | [[Category: Marcuccio, S M.]] | ||
[[Category: Ooi, H | [[Category: Ooi, H C.]] | ||
[[Category: Pilling, P | [[Category: Pilling, P A.]] | ||
[[Category: Smith, B | [[Category: Smith, B J.]] | ||
[[Category: PYR]] | [[Category: PYR]] | ||
[[Category: aldolase]] | [[Category: aldolase]] | ||
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[[Category: oxo-acid lyase]] | [[Category: oxo-acid lyase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:37:41 2008'' | ||
