1fhz: Difference between revisions
New page: left|200px<br /><applet load="1fhz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fhz, resolution 2.20Å" /> '''PSORALEN CROSS-LINKE... |
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[[Image:1fhz.gif|left|200px]]<br /><applet load="1fhz" size=" | [[Image:1fhz.gif|left|200px]]<br /><applet load="1fhz" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1fhz, resolution 2.20Å" /> | caption="1fhz, resolution 2.20Å" /> | ||
'''PSORALEN CROSS-LINKED D(CCGGTACCGG) FORMS HOLLIDAY JUNCTION'''<br /> | '''PSORALEN CROSS-LINKED D(CCGGTACCGG) FORMS HOLLIDAY JUNCTION'''<br /> | ||
==Overview== | ==Overview== | ||
The single-crystal structures are presented for two DNA sequences with the | The single-crystal structures are presented for two DNA sequences with the thymine bases covalently cross-linked across the complementary strands by 4'-hydroxymethyl-4,5',8-trimethylpsoralen (HMT). The HMT-adduct of d(CCGCTAGCGG) forms a psoralen-induced Holliday junction, showing for the first time the effect of this important class of chemotheraputics on the structure of the recombination intermediate. In contrast, HMT-d(CCGGTACCGG) forms a sequence-dependent junction. In both structures, the DNA duplex is highly distorted at the thymine base linked to the six-member pyrone ring of the drug. The psoralen cross-link defines the intramolecular interactions of the drug-induced junction, while the sequence-dependent structure is nearly identical to the native Holliday junction of d(CCGGTACCGG) alone. The two structures contrast the effects of drug- and sequence-dependent interactions on the structure of a Holliday junction, suggesting a role for psoralen in the mechanism to initiate repair of psoralen-lesions in mammalian DNA. | ||
==About this Structure== | ==About this Structure== | ||
1FHZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with PSO as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1FHZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=PSO:'>PSO</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FHZ OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Alberti, M.]] | [[Category: Alberti, M.]] | ||
[[Category: Eichman, B | [[Category: Eichman, B F.]] | ||
[[Category: Hearst, J | [[Category: Hearst, J E.]] | ||
[[Category: Ho, P | [[Category: Ho, P S.]] | ||
[[Category: Mooers, B | [[Category: Mooers, B H.M.]] | ||
[[Category: PSO]] | [[Category: PSO]] | ||
[[Category: cross-linked dna]] | [[Category: cross-linked dna]] | ||
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[[Category: psoralen]] | [[Category: psoralen]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:38:50 2008'' | ||
Revision as of 10:38, 21 February 2008
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PSORALEN CROSS-LINKED D(CCGGTACCGG) FORMS HOLLIDAY JUNCTION
Overview
The single-crystal structures are presented for two DNA sequences with the thymine bases covalently cross-linked across the complementary strands by 4'-hydroxymethyl-4,5',8-trimethylpsoralen (HMT). The HMT-adduct of d(CCGCTAGCGG) forms a psoralen-induced Holliday junction, showing for the first time the effect of this important class of chemotheraputics on the structure of the recombination intermediate. In contrast, HMT-d(CCGGTACCGG) forms a sequence-dependent junction. In both structures, the DNA duplex is highly distorted at the thymine base linked to the six-member pyrone ring of the drug. The psoralen cross-link defines the intramolecular interactions of the drug-induced junction, while the sequence-dependent structure is nearly identical to the native Holliday junction of d(CCGGTACCGG) alone. The two structures contrast the effects of drug- and sequence-dependent interactions on the structure of a Holliday junction, suggesting a role for psoralen in the mechanism to initiate repair of psoralen-lesions in mammalian DNA.
About this Structure
1FHZ is a Protein complex structure of sequences from [1] with PSO as ligand. Full crystallographic information is available from OCA.
Reference
The crystal structures of psoralen cross-linked DNAs: drug-dependent formation of Holliday junctions., Eichman BF, Mooers BH, Alberti M, Hearst JE, Ho PS, J Mol Biol. 2001 Apr 20;308(1):15-26. PMID:11302703
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