1flo: Difference between revisions

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==Overview==

The crystal structure of a Flp recombinase tetramer bound to a Holliday, junction intermediate has been determined at 2.65 A resolution. Only one, of Flp's two domains, containing the active site, is structurally related, to other lambda integrase family site-specific recombinases, such as Cre., The Flp active site differs, however, in that the helix containing the, nucleophilic tyrosine is domain swapped, such that it cuts its DNA target, in trans. The Flp tetramer displays pseudo four-fold symmetry matching, that of the square planar Holliday junction substrate. This tetramer is, stabilized by additional novel trans interactions among monomers. The, structure illustrates how mechanistic unity is maintained on a chemical, level while allowing for substantial variation on the structural level, within a family of enzymes.

The crystal structure of a Flp recombinase tetramer bound to a Holliday junction intermediate has been determined at 2.65 A resolution. Only one of Flp's two domains, containing the active site, is structurally related to other lambda integrase family site-specific recombinases, such as Cre. The Flp active site differs, however, in that the helix containing the nucleophilic tyrosine is domain swapped, such that it cuts its DNA target in trans. The Flp tetramer displays pseudo four-fold symmetry matching that of the square planar Holliday junction substrate. This tetramer is stabilized by additional novel trans interactions among monomers. The structure illustrates how mechanistic unity is maintained on a chemical level while allowing for substantial variation on the structural level within a family of enzymes.

==About this Structure==

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[[Category: Single protein]]

[[Category: Chen, Y.]]

[[Category: Cox, M.M.]]

[[Category: Cox, M M.]]

[[Category: Iype, L.E.]]

[[Category: Iype, L E.]]

[[Category: Narendra, U.]]

[[Category: Rice, P.A.]]

[[Category: Rice, P A.]]

[[Category: PHS]]

[[Category: domain-swapping]]

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[[Category: tyrosine recombinase]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-The crystal structure of a Flp recombinase tetramer bound to a Holliday, junction intermediate has been determined at 2.65 A resolution. Only one, of Flp's two domains, containing the active site, is structurally related, to other lambda integrase family site-specific recombinases, such as Cre., The Flp active site differs, however, in that the helix containing the, nucleophilic tyrosine is domain swapped, such that it cuts its DNA target, in trans. The Flp tetramer displays pseudo four-fold symmetry matching, that of the square planar Holliday junction substrate. This tetramer is, stabilized by additional novel trans interactions among monomers. The, structure illustrates how mechanistic unity is maintained on a chemical, level while allowing for substantial variation on the structural level, within a family of enzymes.
+The crystal structure of a Flp recombinase tetramer bound to a Holliday junction intermediate has been determined at 2.65 A resolution. Only one of Flp's two domains, containing the active site, is structurally related to other lambda integrase family site-specific recombinases, such as Cre. The Flp active site differs, however, in that the helix containing the nucleophilic tyrosine is domain swapped, such that it cuts its DNA target in trans. The Flp tetramer displays pseudo four-fold symmetry matching that of the square planar Holliday junction substrate. This tetramer is stabilized by additional novel trans interactions among monomers. The structure illustrates how mechanistic unity is maintained on a chemical level while allowing for substantial variation on the structural level within a family of enzymes.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Chen, Y.]]
-[[Category: Cox, M.M.]]
+[[Category: Cox, M M.]]
-[[Category: Iype, L.E.]]
+[[Category: Iype, L E.]]
 [[Category: Narendra, U.]]
-[[Category: Rice, P.A.]]
+[[Category: Rice, P A.]]
 [[Category: PHS]]
 [[Category: domain-swapping]]
@@ Line 24: / Line 24: @@
 [[Category: tyrosine recombinase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 09:39:56 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:39:57 2008''