1fs8: Difference between revisions

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[[Image:1fs8.jpg|left|200px]]<br /><applet load="1fs8" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1fs8.jpg|left|200px]]<br /><applet load="1fs8" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1fs8, resolution 1.60Å" />

'''CYTOCHROME C NITRITE REDUCTASE FROM WOLINELLA SUCCINOGENES-SULFATE COMPLEX'''<br />

==Overview==

Cytochrome c nitrite reductase catalyzes the 6-electron reduction of, nitrite to ammonia. This second part of the respiratory pathway of nitrate, ammonification is a key step in the biological nitrogen cycle. The x-ray, structure of the enzyme from the epsilon-proteobacterium Wolinella, succinogenes has been solved to a resolution of 1.6 A. It is a pentaheme, c-type cytochrome whose heme groups are packed in characteristic motifs, that also occur in other multiheme cytochromes. Structures of W., succinogenes nitrite reductase have been obtained with water bound to the, active site heme iron as well as complexes with two inhibitors, sulfate, and azide, whose binding modes and inhibitory functions differ, significantly. Cytochrome c nitrite reductase is part of a highly, optimized respiratory system found in a wide range of Gram-negative, bacteria. It reduces both anionic and neutral substrates at the distal, side of a lysine-coordinated high-spin heme group, which is accessible, through two different channels, allowing for a guided flow of reaction, educt and product. Based on sequence comparison and secondary structure, prediction, we have demonstrated that cytochrome c nitrite reductases, constitute a protein family of high structural similarity.

Cytochrome c nitrite reductase catalyzes the 6-electron reduction of nitrite to ammonia. This second part of the respiratory pathway of nitrate ammonification is a key step in the biological nitrogen cycle. The x-ray structure of the enzyme from the epsilon-proteobacterium Wolinella succinogenes has been solved to a resolution of 1.6 A. It is a pentaheme c-type cytochrome whose heme groups are packed in characteristic motifs that also occur in other multiheme cytochromes. Structures of W. succinogenes nitrite reductase have been obtained with water bound to the active site heme iron as well as complexes with two inhibitors, sulfate and azide, whose binding modes and inhibitory functions differ significantly. Cytochrome c nitrite reductase is part of a highly optimized respiratory system found in a wide range of Gram-negative bacteria. It reduces both anionic and neutral substrates at the distal side of a lysine-coordinated high-spin heme group, which is accessible through two different channels, allowing for a guided flow of reaction educt and product. Based on sequence comparison and secondary structure prediction, we have demonstrated that cytochrome c nitrite reductases constitute a protein family of high structural similarity.

==About this Structure==

1FS8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes] with CA, Y1, SO4, ACT and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1FS8 OCA].

1FS8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=Y1:'>Y1</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=ACT:'>ACT</scene> and <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FS8 OCA].

==Reference==

Line 16:

[[Category: Huber, R.]]

[[Category: Kroeger, A.]]

[[Category: Kroneck, P.M.H.]]

[[Category: Kroneck, P M.H.]]

[[Category: Messerschmidt, A.]]

[[Category: Simon, J.]]

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[[Category: c-type cytochrome]]

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@@ Line 1: / Line 1: @@
-[[Image:1fs8.jpg|left|200px]]<br /><applet load="1fs8" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1fs8.jpg|left|200px]]<br /><applet load="1fs8" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1fs8, resolution 1.60&Aring;" />
 '''CYTOCHROME C NITRITE REDUCTASE FROM WOLINELLA SUCCINOGENES-SULFATE COMPLEX'''<br />
 ==Overview==
-Cytochrome c nitrite reductase catalyzes the 6-electron reduction of, nitrite to ammonia. This second part of the respiratory pathway of nitrate, ammonification is a key step in the biological nitrogen cycle. The x-ray, structure of the enzyme from the epsilon-proteobacterium Wolinella, succinogenes has been solved to a resolution of 1.6 A. It is a pentaheme, c-type cytochrome whose heme groups are packed in characteristic motifs, that also occur in other multiheme cytochromes. Structures of W., succinogenes nitrite reductase have been obtained with water bound to the, active site heme iron as well as complexes with two inhibitors, sulfate, and azide, whose binding modes and inhibitory functions differ, significantly. Cytochrome c nitrite reductase is part of a highly, optimized respiratory system found in a wide range of Gram-negative, bacteria. It reduces both anionic and neutral substrates at the distal, side of a lysine-coordinated high-spin heme group, which is accessible, through two different channels, allowing for a guided flow of reaction, educt and product. Based on sequence comparison and secondary structure, prediction, we have demonstrated that cytochrome c nitrite reductases, constitute a protein family of high structural similarity.
+Cytochrome c nitrite reductase catalyzes the 6-electron reduction of nitrite to ammonia. This second part of the respiratory pathway of nitrate ammonification is a key step in the biological nitrogen cycle. The x-ray structure of the enzyme from the epsilon-proteobacterium Wolinella succinogenes has been solved to a resolution of 1.6 A. It is a pentaheme c-type cytochrome whose heme groups are packed in characteristic motifs that also occur in other multiheme cytochromes. Structures of W. succinogenes nitrite reductase have been obtained with water bound to the active site heme iron as well as complexes with two inhibitors, sulfate and azide, whose binding modes and inhibitory functions differ significantly. Cytochrome c nitrite reductase is part of a highly optimized respiratory system found in a wide range of Gram-negative bacteria. It reduces both anionic and neutral substrates at the distal side of a lysine-coordinated high-spin heme group, which is accessible through two different channels, allowing for a guided flow of reaction educt and product. Based on sequence comparison and secondary structure prediction, we have demonstrated that cytochrome c nitrite reductases constitute a protein family of high structural similarity.
 ==About this Structure==
-FS8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes] with CA, Y1, SO4, ACT and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FS8 OCA].
+FS8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=Y1:'>Y1</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=ACT:'>ACT</scene> and <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FS8 OCA].
 ==Reference==
@@ Line 16: / Line 16: @@
 [[Category: Huber, R.]]
 [[Category: Kroeger, A.]]
-[[Category: Kroneck, P.M.H.]]
+[[Category: Kroneck, P M.H.]]
 [[Category: Messerschmidt, A.]]
 [[Category: Simon, J.]]
@@ Line 27: / Line 27: @@
 [[Category: c-type cytochrome]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:13:54 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:42:06 2008''