1g1j: Difference between revisions
New page: left|200px<br /><applet load="1g1j" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g1j, resolution 1.86Å" /> '''CRYSTAL STRUCTURE OF... |
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[[Image:1g1j.gif|left|200px]]<br /><applet load="1g1j" size=" | [[Image:1g1j.gif|left|200px]]<br /><applet load="1g1j" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1g1j, resolution 1.86Å" /> | caption="1g1j, resolution 1.86Å" /> | ||
'''CRYSTAL STRUCTURE OF THE OLIGOMERIZATION DOMAIN FROM ROTAVIRUS NSP4'''<br /> | '''CRYSTAL STRUCTURE OF THE OLIGOMERIZATION DOMAIN FROM ROTAVIRUS NSP4'''<br /> | ||
==Overview== | ==Overview== | ||
During the maturation of rotaviral particles, non-structural protein 4 | During the maturation of rotaviral particles, non-structural protein 4 (NSP4) plays a critical role in the translocation of the immature capsid into the lumen of the endoplasmic reticulum. Full-length NSP4 and a 22 amino acid peptide (NSP4(114-135)) derived from this protein have been shown to induce diarrhea in young mice in an age-dependent manner, and may therefore be the agent responsible for rotavirally-induced symptoms. We have determined the crystal structure of the oligomerization domain of NSP4 which spans residues 95 to 137 (NSP4(95-137)). NSP4(95-137) self-associates into a parallel, tetrameric coiled-coil, with the hydrophobic core interrupted by three polar layers occupying a and d-heptad positions. Side-chains from two consecutive polar layers, consisting of four Gln123 and two of the four Glu120 residues, coordinate a divalent cation. Two independent structures built from MAD-phased data indicated the presence of a strontium and calcium ion bound at this site, respectively. This metal-binding site appears to play an important role in stabilizing the homo-tetramer, which has implications for the engagement of NSP4 as an enterotoxin. | ||
==About this Structure== | ==About this Structure== | ||
1G1J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with SR as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1G1J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SR:'>SR</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G1J OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of the oligomerization domain of NSP4 from rotavirus reveals a core metal-binding site., Bowman GD, Nodelman IM, Levy O, Lin SL, Tian P, Zamb TJ, Udem SA, Venkataraghavan B, Schutt CE, J Mol Biol. 2000 Dec 15;304(5):861-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11124032 11124032] | Crystal structure of the oligomerization domain of NSP4 from rotavirus reveals a core metal-binding site., Bowman GD, Nodelman IM, Levy O, Lin SL, Tian P, Zamb TJ, Udem SA, Venkataraghavan B, Schutt CE, J Mol Biol. 2000 Dec 15;304(5):861-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11124032 11124032] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bowman, G | [[Category: Bowman, G D.]] | ||
[[Category: Nodelman, I | [[Category: Nodelman, I M.]] | ||
[[Category: Schutt, C | [[Category: Schutt, C E.]] | ||
[[Category: SR]] | [[Category: SR]] | ||
[[Category: cation]] | [[Category: cation]] | ||
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[[Category: strontium]] | [[Category: strontium]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:45:00 2008'' | ||
Revision as of 10:45, 21 February 2008
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CRYSTAL STRUCTURE OF THE OLIGOMERIZATION DOMAIN FROM ROTAVIRUS NSP4
Overview
During the maturation of rotaviral particles, non-structural protein 4 (NSP4) plays a critical role in the translocation of the immature capsid into the lumen of the endoplasmic reticulum. Full-length NSP4 and a 22 amino acid peptide (NSP4(114-135)) derived from this protein have been shown to induce diarrhea in young mice in an age-dependent manner, and may therefore be the agent responsible for rotavirally-induced symptoms. We have determined the crystal structure of the oligomerization domain of NSP4 which spans residues 95 to 137 (NSP4(95-137)). NSP4(95-137) self-associates into a parallel, tetrameric coiled-coil, with the hydrophobic core interrupted by three polar layers occupying a and d-heptad positions. Side-chains from two consecutive polar layers, consisting of four Gln123 and two of the four Glu120 residues, coordinate a divalent cation. Two independent structures built from MAD-phased data indicated the presence of a strontium and calcium ion bound at this site, respectively. This metal-binding site appears to play an important role in stabilizing the homo-tetramer, which has implications for the engagement of NSP4 as an enterotoxin.
About this Structure
1G1J is a Single protein structure of sequence from [1] with SR as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of the oligomerization domain of NSP4 from rotavirus reveals a core metal-binding site., Bowman GD, Nodelman IM, Levy O, Lin SL, Tian P, Zamb TJ, Udem SA, Venkataraghavan B, Schutt CE, J Mol Biol. 2000 Dec 15;304(5):861-71. PMID:11124032
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