1g72: Difference between revisions
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New page: left|200px<br /><applet load="1g72" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g72, resolution 1.90Å" /> '''CATALYTIC MECHANISM ... |
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[[Image:1g72.jpg|left|200px]]<br /><applet load="1g72" size=" | [[Image:1g72.jpg|left|200px]]<br /><applet load="1g72" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1g72, resolution 1.90Å" /> | caption="1g72, resolution 1.90Å" /> | ||
'''CATALYTIC MECHANISM OF QUINOPROTEIN METHANOL DEHYDROGENASE: A THEORETICAL AND X-RAY CRYSTALLOGRAPHIC INVESTIGATION'''<br /> | '''CATALYTIC MECHANISM OF QUINOPROTEIN METHANOL DEHYDROGENASE: A THEORETICAL AND X-RAY CRYSTALLOGRAPHIC INVESTIGATION'''<br /> | ||
==Overview== | ==Overview== | ||
The catalytic mechanism of the reductive half reaction of the quinoprotein | The catalytic mechanism of the reductive half reaction of the quinoprotein methanol dehydrogenase (MDH) is believed to proceed either through a hemiketal intermediate or by direct transfer of a hydride ion from the substrate methyl group to the cofactor, pyrroloquinoline quinone (PQQ). A crystal structure of the enzyme-substrate complex of a similar quinoprotein, glucose dehydrogenase, has recently been reported that strongly favors the hydride transfer mechanism in that enzyme. A theoretical analysis and an improved refinement of the 1.9-A resolution crystal structure of MDH from Methylophilus methylotrophus W3A1 in the presence of methanol, reported earlier, indicates that the observed tetrahedral configuration of the C-5 atom of PQQ in that study represents the C-5-reduced form of the cofactor and lends support for a hydride transfer mechanism for MDH. | ||
==About this Structure== | ==About this Structure== | ||
1G72 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Methylophilus_methylotrophus Methylophilus methylotrophus] with CA and PQQ as [http://en.wikipedia.org/wiki/ligands ligands]. This structure | 1G72 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Methylophilus_methylotrophus Methylophilus methylotrophus] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=PQQ:'>PQQ</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure supersedes the now removed PDB entry 1B2N. Active as [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase_(acceptor) Alcohol dehydrogenase (acceptor)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.99.8 1.1.99.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G72 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Methylophilus methylotrophus]] | [[Category: Methylophilus methylotrophus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Bruice, T | [[Category: Bruice, T C.]] | ||
[[Category: Chen, Z.]] | [[Category: Chen, Z.]] | ||
[[Category: Mathews, F | [[Category: Mathews, F S.]] | ||
[[Category: Xia, Z.]] | [[Category: Xia, Z.]] | ||
[[Category: Zheng, Y.]] | [[Category: Zheng, Y.]] | ||
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[[Category: quinoprotein]] | [[Category: quinoprotein]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:46:51 2008'' | ||
