1gnu: Difference between revisions

Line 4:

==Overview==

The gamma-aminobutyric acid receptor type A (GABA(A)) receptor-associated, protein (GABARAP) has been reported to mediate the interaction between the, GABA(A) receptor and microtubules. We present the three-dimensional, structure of GABARAP obtained by x-ray diffraction at 1.75 A resolution., The structure was determined by molecular replacement using the structure, of the homologous protein GATE-16. NMR spectroscopy of isotope-labeled, GABARAP showed the structure in solution to be compatible with the overall, fold but showed evidence of conformation heterogeneity that is not, apparent in the crystal structure. We assessed the binding of GABARAP to, peptides derived from reported binding partner proteins, including the, M3-M4 loop of the gamma2 subunit of the GABA(A) receptor and the acidic, carboxyl-terminal tails of human alpha- and beta-tubulin. There is a small, area of concentrated positive charge on one surface of GABARAP, which we, found interacts weakly with all peptides tested, but we found no evidence, for specific binding to the proposed physiological target peptides. These, results are compatible with a more general role in membrane targeting and, transportation for the GABARAP family of proteins.

The gamma-aminobutyric acid receptor type A (GABA(A)) receptor-associated protein (GABARAP) has been reported to mediate the interaction between the GABA(A) receptor and microtubules. We present the three-dimensional structure of GABARAP obtained by x-ray diffraction at 1.75 A resolution. The structure was determined by molecular replacement using the structure of the homologous protein GATE-16. NMR spectroscopy of isotope-labeled GABARAP showed the structure in solution to be compatible with the overall fold but showed evidence of conformation heterogeneity that is not apparent in the crystal structure. We assessed the binding of GABARAP to peptides derived from reported binding partner proteins, including the M3-M4 loop of the gamma2 subunit of the GABA(A) receptor and the acidic carboxyl-terminal tails of human alpha- and beta-tubulin. There is a small area of concentrated positive charge on one surface of GABARAP, which we found interacts weakly with all peptides tested, but we found no evidence for specific binding to the proposed physiological target peptides. These results are compatible with a more general role in membrane targeting and transportation for the GABARAP family of proteins.

==About this Structure==

Line 13:

[[Category: Homo sapiens]]

[[Category: Single protein]]

[[Category: Driscoll, P.C.]]

[[Category: Driscoll, P C.]]

[[Category: Harris, R.]]

[[Category: Keep, N.H.]]

[[Category: Keep, N H.]]

[[Category: Knight, D.]]

[[Category: Moss, S.]]

Line 23:

[[Category: ubiquitin-like]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun~~ Feb ~~3 09~~:41:16 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:52:04 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 4: / Line 4: @@
 ==Overview==
-The gamma-aminobutyric acid receptor type A (GABA(A)) receptor-associated, protein (GABARAP) has been reported to mediate the interaction between the, GABA(A) receptor and microtubules. We present the three-dimensional, structure of GABARAP obtained by x-ray diffraction at 1.75 A resolution., The structure was determined by molecular replacement using the structure, of the homologous protein GATE-16. NMR spectroscopy of isotope-labeled, GABARAP showed the structure in solution to be compatible with the overall, fold but showed evidence of conformation heterogeneity that is not, apparent in the crystal structure. We assessed the binding of GABARAP to, peptides derived from reported binding partner proteins, including the, M3-M4 loop of the gamma2 subunit of the GABA(A) receptor and the acidic, carboxyl-terminal tails of human alpha- and beta-tubulin. There is a small, area of concentrated positive charge on one surface of GABARAP, which we, found interacts weakly with all peptides tested, but we found no evidence, for specific binding to the proposed physiological target peptides. These, results are compatible with a more general role in membrane targeting and, transportation for the GABARAP family of proteins.
+The gamma-aminobutyric acid receptor type A (GABA(A)) receptor-associated protein (GABARAP) has been reported to mediate the interaction between the GABA(A) receptor and microtubules. We present the three-dimensional structure of GABARAP obtained by x-ray diffraction at 1.75 A resolution. The structure was determined by molecular replacement using the structure of the homologous protein GATE-16. NMR spectroscopy of isotope-labeled GABARAP showed the structure in solution to be compatible with the overall fold but showed evidence of conformation heterogeneity that is not apparent in the crystal structure. We assessed the binding of GABARAP to peptides derived from reported binding partner proteins, including the M3-M4 loop of the gamma2 subunit of the GABA(A) receptor and the acidic carboxyl-terminal tails of human alpha- and beta-tubulin. There is a small area of concentrated positive charge on one surface of GABARAP, which we found interacts weakly with all peptides tested, but we found no evidence for specific binding to the proposed physiological target peptides. These results are compatible with a more general role in membrane targeting and transportation for the GABARAP family of proteins.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
-[[Category: Driscoll, P.C.]]
+[[Category: Driscoll, P C.]]
 [[Category: Harris, R.]]
-[[Category: Keep, N.H.]]
+[[Category: Keep, N H.]]
 [[Category: Knight, D.]]
 [[Category: Moss, S.]]
@@ Line 23: / Line 23: @@
 [[Category: ubiquitin-like]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 09:41:16 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:52:04 2008''