1gql: Difference between revisions

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==Overview==

Alpha-glucuronidases, components of an ensemble of enzymes central to the, recycling of photosynthetic biomass, remove the alpha-1,2 linked, 4-O-methyl glucuronic acid from xylans. The structure of the, alpha-glucuronidase, GlcA67A, from Pseudomonas cellulosa reveals three, domains, the central of which is a (beta/alpha)(8) barrel housing the, catalytic apparatus. Complexes of the enzyme with the individual reaction, products, either xylobiose or glucuronic acid, and the ternary complex of, both glucuronic acid and xylotriose reveal a "blind" pocket which selects, for short decorated xylooligosaccharides substituted with the uronic acid, at their nonreducing end, consistent with kinetic data. The catalytic, center reveals a constellation of carboxylates; Glu292 is poised to, provide protonic assistance to leaving group departure with Glu393 and, Asp365 both appropriately positioned to provide base-catalyzed assistance, for inverting nucleophilic attack by water.

Alpha-glucuronidases, components of an ensemble of enzymes central to the recycling of photosynthetic biomass, remove the alpha-1,2 linked 4-O-methyl glucuronic acid from xylans. The structure of the alpha-glucuronidase, GlcA67A, from Pseudomonas cellulosa reveals three domains, the central of which is a (beta/alpha)(8) barrel housing the catalytic apparatus. Complexes of the enzyme with the individual reaction products, either xylobiose or glucuronic acid, and the ternary complex of both glucuronic acid and xylotriose reveal a "blind" pocket which selects for short decorated xylooligosaccharides substituted with the uronic acid at their nonreducing end, consistent with kinetic data. The catalytic center reveals a constellation of carboxylates; Glu292 is poised to provide protonic assistance to leaving group departure with Glu393 and Asp365 both appropriately positioned to provide base-catalyzed assistance for inverting nucleophilic attack by water.

==About this Structure==

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[[Category: Cellvibrio japonicus]]

[[Category: Single protein]]

[[Category: Davies, G.J.]]

[[Category: Davies, G J.]]

[[Category: Gilbert, H.J.]]

[[Category: Gilbert, H J.]]

[[Category: Nagy, T.]]

[[Category: Nurizzo, D.]]

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[[Category: xylotriose]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-Alpha-glucuronidases, components of an ensemble of enzymes central to the, recycling of photosynthetic biomass, remove the alpha-1,2 linked, 4-O-methyl glucuronic acid from xylans. The structure of the, alpha-glucuronidase, GlcA67A, from Pseudomonas cellulosa reveals three, domains, the central of which is a (beta/alpha)(8) barrel housing the, catalytic apparatus. Complexes of the enzyme with the individual reaction, products, either xylobiose or glucuronic acid, and the ternary complex of, both glucuronic acid and xylotriose reveal a "blind" pocket which selects, for short decorated xylooligosaccharides substituted with the uronic acid, at their nonreducing end, consistent with kinetic data. The catalytic, center reveals a constellation of carboxylates; Glu292 is poised to, provide protonic assistance to leaving group departure with Glu393 and, Asp365 both appropriately positioned to provide base-catalyzed assistance, for inverting nucleophilic attack by water.
+Alpha-glucuronidases, components of an ensemble of enzymes central to the recycling of photosynthetic biomass, remove the alpha-1,2 linked 4-O-methyl glucuronic acid from xylans. The structure of the alpha-glucuronidase, GlcA67A, from Pseudomonas cellulosa reveals three domains, the central of which is a (beta/alpha)(8) barrel housing the catalytic apparatus. Complexes of the enzyme with the individual reaction products, either xylobiose or glucuronic acid, and the ternary complex of both glucuronic acid and xylotriose reveal a "blind" pocket which selects for short decorated xylooligosaccharides substituted with the uronic acid at their nonreducing end, consistent with kinetic data. The catalytic center reveals a constellation of carboxylates; Glu292 is poised to provide protonic assistance to leaving group departure with Glu393 and Asp365 both appropriately positioned to provide base-catalyzed assistance for inverting nucleophilic attack by water.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Cellvibrio japonicus]]
 [[Category: Single protein]]
-[[Category: Davies, G.J.]]
+[[Category: Davies, G J.]]
-[[Category: Gilbert, H.J.]]
+[[Category: Gilbert, H J.]]
 [[Category: Nagy, T.]]
 [[Category: Nurizzo, D.]]
@@ Line 28: / Line 28: @@
 [[Category: xylotriose]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 09:41:57 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:52:54 2008''