1gvn: Difference between revisions

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==Overview==

Programmed cell death in prokaryotes is frequently found as, postsegregational killing. It relies on antitoxin/toxin systems that, secure stable inheritance of low and medium copy number plasmids during, cell division and kill cells that have lost the plasmid. The, broad-host-range, low-copy-number plasmid pSM19035 from Streptococcus, pyogenes carries the genes encoding the antitoxin/toxin system, epsilon/zeta and antibiotic resistance proteins, among others. The crystal, structure of the biologically nontoxic epsilon(2)zeta(2) protein complex, at a 1.95-A resolution and site-directed mutagenesis showed that free zeta, acts as phosphotransferase by using ATPGTP. In epsilon(2)zeta(2), the, toxin zeta is inactivated because the N-terminal helix of the antitoxin, epsilon blocks the ATPGTP-binding site. To our knowledge, this is the, first prokaryotic postsegregational killing system that has been entirely, structurally characterized.

Programmed cell death in prokaryotes is frequently found as postsegregational killing. It relies on antitoxin/toxin systems that secure stable inheritance of low and medium copy number plasmids during cell division and kill cells that have lost the plasmid. The broad-host-range, low-copy-number plasmid pSM19035 from Streptococcus pyogenes carries the genes encoding the antitoxin/toxin system epsilon/zeta and antibiotic resistance proteins, among others. The crystal structure of the biologically nontoxic epsilon(2)zeta(2) protein complex at a 1.95-A resolution and site-directed mutagenesis showed that free zeta acts as phosphotransferase by using ATPGTP. In epsilon(2)zeta(2), the toxin zeta is inactivated because the N-terminal helix of the antitoxin epsilon blocks the ATPGTP-binding site. To our knowledge, this is the first prokaryotic postsegregational killing system that has been entirely structurally characterized.

==About this Structure==

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[[Category: Protein complex]]

[[Category: Streptococcus pyogenes]]

[[Category: Alonso, J.C.]]

[[Category: Alonso, J C.]]

[[Category: Meinhart, A.]]

[[Category: Saenger, W.]]

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[[Category: postsegregational killing system]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-Programmed cell death in prokaryotes is frequently found as, postsegregational killing. It relies on antitoxin/toxin systems that, secure stable inheritance of low and medium copy number plasmids during, cell division and kill cells that have lost the plasmid. The, broad-host-range, low-copy-number plasmid pSM19035 from Streptococcus, pyogenes carries the genes encoding the antitoxin/toxin system, epsilon/zeta and antibiotic resistance proteins, among others. The crystal, structure of the biologically nontoxic epsilon(2)zeta(2) protein complex, at a 1.95-A resolution and site-directed mutagenesis showed that free zeta, acts as phosphotransferase by using ATPGTP. In epsilon(2)zeta(2), the, toxin zeta is inactivated because the N-terminal helix of the antitoxin, epsilon blocks the ATPGTP-binding site. To our knowledge, this is the, first prokaryotic postsegregational killing system that has been entirely, structurally characterized.
+Programmed cell death in prokaryotes is frequently found as postsegregational killing. It relies on antitoxin/toxin systems that secure stable inheritance of low and medium copy number plasmids during cell division and kill cells that have lost the plasmid. The broad-host-range, low-copy-number plasmid pSM19035 from Streptococcus pyogenes carries the genes encoding the antitoxin/toxin system epsilon/zeta and antibiotic resistance proteins, among others. The crystal structure of the biologically nontoxic epsilon(2)zeta(2) protein complex at a 1.95-A resolution and site-directed mutagenesis showed that free zeta acts as phosphotransferase by using ATPGTP. In epsilon(2)zeta(2), the toxin zeta is inactivated because the N-terminal helix of the antitoxin epsilon blocks the ATPGTP-binding site. To our knowledge, this is the first prokaryotic postsegregational killing system that has been entirely structurally characterized.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Protein complex]]
 [[Category: Streptococcus pyogenes]]
-[[Category: Alonso, J.C.]]
+[[Category: Alonso, J C.]]
 [[Category: Meinhart, A.]]
 [[Category: Saenger, W.]]
@@ Line 21: / Line 21: @@
 [[Category: postsegregational killing system]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 09:43:09 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:54:29 2008''