1gxn: Difference between revisions
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New page: left|200px<br /><applet load="1gxn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gxn, resolution 1.50Å" /> '''FAMILY 10 POLYSACCHA... |
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[[Image:1gxn.gif|left|200px]]<br /><applet load="1gxn" size=" | [[Image:1gxn.gif|left|200px]]<br /><applet load="1gxn" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1gxn, resolution 1.50Å" /> | caption="1gxn, resolution 1.50Å" /> | ||
'''FAMILY 10 POLYSACCHARIDE LYASE FROM CELLVIBRIO CELLULOSA'''<br /> | '''FAMILY 10 POLYSACCHARIDE LYASE FROM CELLVIBRIO CELLULOSA'''<br /> | ||
==Overview== | ==Overview== | ||
Enzyme-catalyzed beta-elimination of sugar uronic acids, exemplified by | Enzyme-catalyzed beta-elimination of sugar uronic acids, exemplified by the degradation of plant cell wall pectins, plays an important role in a wide spectrum of biological processes ranging from the recycling of plant biomass through to pathogen virulence. The three-dimensional crystal structure of the catalytic module of a "family PL-10" polysaccharide lyase, Pel10Acm from Cellvibrio japonicus, solved at a resolution of 1.3 A, reveals a new polysaccharide lyase fold and is the first example of a polygalacturonic acid lyase that does not exhibit the "parallel beta-helix" topology. The "Michaelis" complex of an inactive mutant in association with the substrate trigalacturonate/Ca2+ reveals the catalytic machinery harnessed by this polygalacturonate lyase, which displays a stunning resemblance, presumably through convergent evolution, to the tetragalacturonic acid complex observed for a structurally unrelated polygalacturonate lyase from family PL-1. Common coordination of the -1 and +1 subsite saccharide carboxylate groups by a protein-liganded Ca2+ ion, the positioning of an arginine catalytic base in close proximity to the alpha-carbon hydrogen and numerous other conserved enzyme-substrate interactions, considered in light of mutagenesis data for both families, suggest a generic polysaccharide anti-beta-elimination mechanism. | ||
==About this Structure== | ==About this Structure== | ||
1GXN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Active as [http://en.wikipedia.org/wiki/Pectate_lyase Pectate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.2 4.2.2.2] Full crystallographic information is available from [http:// | 1GXN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Active as [http://en.wikipedia.org/wiki/Pectate_lyase Pectate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.2 4.2.2.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GXN OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Pectate lyase]] | [[Category: Pectate lyase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Black, G | [[Category: Black, G W.]] | ||
[[Category: Brown, I | [[Category: Brown, I E.]] | ||
[[Category: Charnock, S | [[Category: Charnock, S J.]] | ||
[[Category: Davies, G | [[Category: Davies, G J.]] | ||
[[Category: Turkenburg, J | [[Category: Turkenburg, J P.]] | ||
[[Category: elimination]] | [[Category: elimination]] | ||
[[Category: lyase]] | [[Category: lyase]] | ||
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[[Category: pectate]] | [[Category: pectate]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:55:06 2008'' | ||
