1h8e: Difference between revisions
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==Overview== | ==Overview== | ||
The crystal structure of a novel aluminium fluoride inhibited form of | The crystal structure of a novel aluminium fluoride inhibited form of bovine mitochondrial F(1)-ATPase has been determined at 2 A resolution. In contrast to all previously determined structures of the bovine enzyme, all three catalytic sites are occupied by nucleotide. The subunit that did not bind nucleotide in previous structures binds ADP and sulfate (mimicking phosphate), and adopts a "half-closed" conformation. This structure probably represents the posthydrolysis, pre-product release step on the catalytic pathway. A catalytic scheme for hydrolysis (and synthesis) at physiological rates and a mechanism for the ATP-driven rotation of the gamma subunit are proposed based on the crystal structures of the bovine enzyme. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Transferred entry: 3 | [[Category: Transferred entry: 3 6.3 14]] | ||
[[Category: Leslie, A | [[Category: Leslie, A G.W.]] | ||
[[Category: Menz, R | [[Category: Menz, R I.]] | ||
[[Category: Walker, J | [[Category: Walker, J E.]] | ||
[[Category: ADP]] | [[Category: ADP]] | ||
[[Category: ALF]] | [[Category: ALF]] | ||
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[[Category: f1fo atp synthase]] | [[Category: f1fo atp synthase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:58:33 2008'' | ||
