1hbx: Difference between revisions

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[[Image:1hbx.gif|left|200px]]<br />

[[Image:1hbx.gif|left|200px]]<br /><applet load="1hbx" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1hbx" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1hbx, resolution 3.15Å" />

'''TERNARY COMPLEX OF SAP-1 AND SRF WITH SPECIFIC SRE DNA'''<br />

==Overview==

The serum response element (SRE) is found in several immediate-early gene, promoters. This DNA sequence is necessary and sufficient for rapid, transcriptional induction of the human c-fos proto-oncogene in response to, stimuli external to the cell. Full activation of the SRE requires the, cooperative binding of a ternary complex factor (TCF) and serum response, factor (SRF) to their specific DNA sites. The X-ray structure of the human, SAP-1-SRF-SRE DNA ternary complex was determined (Protein Data Bank code, 1hbx). It shows SAP-1 TCF bound to SRF through interactions between the, SAP-1 B-box and SRF MADS domain in addition to contacts between their, respective DNA-binding motifs. The SAP-1 B-box is part of a flexible, linker of which 21 amino acids become ordered upon ternary complex, formation. Comparison with a similar region from the yeast, MATalpha2-MCM1-DNA complex suggests a common binding motif through which, MADS-box proteins may interact with additional factors such as Fli-1.

The serum response element (SRE) is found in several immediate-early gene promoters. This DNA sequence is necessary and sufficient for rapid transcriptional induction of the human c-fos proto-oncogene in response to stimuli external to the cell. Full activation of the SRE requires the cooperative binding of a ternary complex factor (TCF) and serum response factor (SRF) to their specific DNA sites. The X-ray structure of the human SAP-1-SRF-SRE DNA ternary complex was determined (Protein Data Bank code 1hbx). It shows SAP-1 TCF bound to SRF through interactions between the SAP-1 B-box and SRF MADS domain in addition to contacts between their respective DNA-binding motifs. The SAP-1 B-box is part of a flexible linker of which 21 amino acids become ordered upon ternary complex formation. Comparison with a similar region from the yeast MATalpha2-MCM1-DNA complex suggests a common binding motif through which MADS-box proteins may interact with additional factors such as Fli-1.

==Disease==

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==About this Structure==

1HBX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1HBX OCA].

1HBX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HBX OCA].

==Reference==

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[[Category: Protein complex]]

[[Category: Hassler, M.]]

[[Category: Richmond, T.J.]]

[[Category: Richmond, T J.]]

[[Category: gene regulation]]

[[Category: serum response factor]]

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[[Category: transcription complex]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov~~ 12 17:15:~~25 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:59:40 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1hbx.gif|left|200px]]<br />
+[[Image:1hbx.gif|left|200px]]<br /><applet load="1hbx" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1hbx" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1hbx, resolution 3.15&Aring;" />
 '''TERNARY COMPLEX OF SAP-1 AND SRF WITH SPECIFIC SRE DNA'''<br />
 ==Overview==
-The serum response element (SRE) is found in several immediate-early gene, promoters. This DNA sequence is necessary and sufficient for rapid, transcriptional induction of the human c-fos proto-oncogene in response to, stimuli external to the cell. Full activation of the SRE requires the, cooperative binding of a ternary complex factor (TCF) and serum response, factor (SRF) to their specific DNA sites. The X-ray structure of the human, SAP-1-SRF-SRE DNA ternary complex was determined (Protein Data Bank code, 1hbx). It shows SAP-1 TCF bound to SRF through interactions between the, SAP-1 B-box and SRF MADS domain in addition to contacts between their, respective DNA-binding motifs. The SAP-1 B-box is part of a flexible, linker of which 21 amino acids become ordered upon ternary complex, formation. Comparison with a similar region from the yeast, MATalpha2-MCM1-DNA complex suggests a common binding motif through which, MADS-box proteins may interact with additional factors such as Fli-1.
+The serum response element (SRE) is found in several immediate-early gene promoters. This DNA sequence is necessary and sufficient for rapid transcriptional induction of the human c-fos proto-oncogene in response to stimuli external to the cell. Full activation of the SRE requires the cooperative binding of a ternary complex factor (TCF) and serum response factor (SRF) to their specific DNA sites. The X-ray structure of the human SAP-1-SRF-SRE DNA ternary complex was determined (Protein Data Bank code 1hbx). It shows SAP-1 TCF bound to SRF through interactions between the SAP-1 B-box and SRF MADS domain in addition to contacts between their respective DNA-binding motifs. The SAP-1 B-box is part of a flexible linker of which 21 amino acids become ordered upon ternary complex formation. Comparison with a similar region from the yeast MATalpha2-MCM1-DNA complex suggests a common binding motif through which MADS-box proteins may interact with additional factors such as Fli-1.
 ==Disease==
@@ Line 11: / Line 10: @@
 ==About this Structure==
-HBX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HBX OCA].
+HBX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HBX OCA].
 ==Reference==
@@ Line 18: / Line 17: @@
 [[Category: Protein complex]]
 [[Category: Hassler, M.]]
-[[Category: Richmond, T.J.]]
+[[Category: Richmond, T J.]]
 [[Category: gene regulation]]
 [[Category: serum response factor]]
@@ Line 24: / Line 23: @@
 [[Category: transcription complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:15:25 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:59:40 2008''