1hcj: Difference between revisions

Line 1:

[[Image:1hcj.jpg|left|200px]]<br /><applet load="1hcj" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1hcj.jpg|left|200px]]<br /><applet load="1hcj" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1hcj, resolution 1.80Å" />

'''PHOTOPRODUCT OF THE WILD-TYPE AEQUOREA VICTORIA GREEN FLUORESCENT PROTEIN'''<br />

==Overview==

Wild type green fluorescent protein (GFP) from Aequorea victoria absorbs, predominantly at 398 nm. Illumination with UV (254 nm) or visible (390 nm), light transforms this state (GFP(398)) into one absorbing at 483 nm, (GFP(483)). Here we show that this photoconversion of GFP is a one-photon, process that is paralleled by decarboxylation of Glu 222. We propose a, mechanism in which decarboxylation is due to electron transfer between the, gamma-carboxylate of Glu 222 and the p-hydroxybenzylidene-imidazolidinone, chromophore of GFP, followed by reverse transfer of an electron and a, proton to the remaining carbon side chain atom of Glu 222. Oxidative, decarboxylation of a gamma-carboxylate represents a new type of, posttranslational modification that may also occur in enzymes with, high-potential reaction intermediates.

Wild type green fluorescent protein (GFP) from Aequorea victoria absorbs predominantly at 398 nm. Illumination with UV (254 nm) or visible (390 nm) light transforms this state (GFP(398)) into one absorbing at 483 nm (GFP(483)). Here we show that this photoconversion of GFP is a one-photon process that is paralleled by decarboxylation of Glu 222. We propose a mechanism in which decarboxylation is due to electron transfer between the gamma-carboxylate of Glu 222 and the p-hydroxybenzylidene-imidazolidinone chromophore of GFP, followed by reverse transfer of an electron and a proton to the remaining carbon side chain atom of Glu 222. Oxidative decarboxylation of a gamma-carboxylate represents a new type of posttranslational modification that may also occur in enzymes with high-potential reaction intermediates.

==About this Structure==

1HCJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1HCJ OCA].

1HCJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HCJ OCA].

==Reference==

Line 14:

[[Category: Single protein]]

[[Category: Gensch, T.]]

[[Category: Hellingwerf, K.J.]]

[[Category: Hellingwerf, K J.]]

[[Category: Johnson, L.]]

[[Category: Thor, J.J.Van.]]

[[Category: Thor, J J.Van.]]

[[Category: beta-barrel]]

[[Category: bioluminescence]]

Line 22:

[[Category: luminescence]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 16~~:31:~~14 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:59:48 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1hcj.jpg|left|200px]]<br /><applet load="1hcj" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1hcj.jpg|left|200px]]<br /><applet load="1hcj" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1hcj, resolution 1.80&Aring;" />
 '''PHOTOPRODUCT OF THE WILD-TYPE AEQUOREA VICTORIA GREEN FLUORESCENT PROTEIN'''<br />
 ==Overview==
-Wild type green fluorescent protein (GFP) from Aequorea victoria absorbs, predominantly at 398 nm. Illumination with UV (254 nm) or visible (390 nm), light transforms this state (GFP(398)) into one absorbing at 483 nm, (GFP(483)). Here we show that this photoconversion of GFP is a one-photon, process that is paralleled by decarboxylation of Glu 222. We propose a, mechanism in which decarboxylation is due to electron transfer between the, gamma-carboxylate of Glu 222 and the p-hydroxybenzylidene-imidazolidinone, chromophore of GFP, followed by reverse transfer of an electron and a, proton to the remaining carbon side chain atom of Glu 222. Oxidative, decarboxylation of a gamma-carboxylate represents a new type of, posttranslational modification that may also occur in enzymes with, high-potential reaction intermediates.
+Wild type green fluorescent protein (GFP) from Aequorea victoria absorbs predominantly at 398 nm. Illumination with UV (254 nm) or visible (390 nm) light transforms this state (GFP(398)) into one absorbing at 483 nm (GFP(483)). Here we show that this photoconversion of GFP is a one-photon process that is paralleled by decarboxylation of Glu 222. We propose a mechanism in which decarboxylation is due to electron transfer between the gamma-carboxylate of Glu 222 and the p-hydroxybenzylidene-imidazolidinone chromophore of GFP, followed by reverse transfer of an electron and a proton to the remaining carbon side chain atom of Glu 222. Oxidative decarboxylation of a gamma-carboxylate represents a new type of posttranslational modification that may also occur in enzymes with high-potential reaction intermediates.
 ==About this Structure==
-HCJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HCJ OCA].
+HCJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HCJ OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Gensch, T.]]
-[[Category: Hellingwerf, K.J.]]
+[[Category: Hellingwerf, K J.]]
 [[Category: Johnson, L.]]
-[[Category: Thor, J.J.Van.]]
+[[Category: Thor, J J.Van.]]
 [[Category: beta-barrel]]
 [[Category: bioluminescence]]
@@ Line 22: / Line 22: @@
 [[Category: luminescence]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:31:14 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:59:48 2008''