1i47: Difference between revisions
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New page: left|200px<br /><applet load="1i47" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i47, resolution 2.1Å" /> '''MOLECULAR AND CRYSTAL... |
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[[Image:1i47.gif|left|200px]]<br /><applet load="1i47" size=" | [[Image:1i47.gif|left|200px]]<br /><applet load="1i47" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1i47, resolution 2.1Å" /> | caption="1i47, resolution 2.1Å" /> | ||
'''MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGAATT(MO4)CGCG): THE WATSON-CRICK TYPE AND WOBBLE N4-METHOXYCYTIDINE/GUANOSINE BASE PAIRS IN B-DNA'''<br /> | '''MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGAATT(MO4)CGCG): THE WATSON-CRICK TYPE AND WOBBLE N4-METHOXYCYTIDINE/GUANOSINE BASE PAIRS IN B-DNA'''<br /> | ||
==Overview== | ==Overview== | ||
To investigate the mutation mechanism of purine transition in DNA damaged | To investigate the mutation mechanism of purine transition in DNA damaged with methoxyamine, a DNA dodecamer with the sequence d(CGCGAATTmo(4)CGCG), where mo(4)C is 2'-deoxy-N(4)-methoxycytidine, has been synthesized and its crystal structure determined. Two dodecamers form a B-form duplex. Electron density maps clearly show that one of the two mo(4)C residues forms a pair with a guanine residue of the opposite strand, the geometry being the canonical Watson-Crick type, and that the other mo(4)C residue forms a wobble pair with the opposite guanine residue. These two pairings are ascribed to the tautomerization of the methoxylated cytosine moieties between the amino and imino forms. | ||
==About this Structure== | ==About this Structure== | ||
1I47 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1I47 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I47 OCA]. | ||
==Reference== | ==Reference== | ||
Crystallographic studies on damaged DNAs: III. N(4)-methoxycytosine can form both Watson-Crick type and wobbled base pairs in a B-form duplex., Hossain MT, Chatake T, Hikima T, Tsunoda M, Sunami T, Ueno Y, Matsuda A, Takenaka A, J Biochem | Crystallographic studies on damaged DNAs: III. N(4)-methoxycytosine can form both Watson-Crick type and wobbled base pairs in a B-form duplex., Hossain MT, Chatake T, Hikima T, Tsunoda M, Sunami T, Ueno Y, Matsuda A, Takenaka A, J Biochem. 2001 Jul;130(1):9-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11432773 11432773] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Chatake, T.]] | [[Category: Chatake, T.]] | ||
[[Category: Hikima, T.]] | [[Category: Hikima, T.]] | ||
[[Category: Hossain, M | [[Category: Hossain, M T.]] | ||
[[Category: Matsuda, A.]] | [[Category: Matsuda, A.]] | ||
[[Category: Sunami, T.]] | [[Category: Sunami, T.]] | ||
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[[Category: n4-methoxycytosine]] | [[Category: n4-methoxycytosine]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:07:49 2008'' | ||
