1i6w: Difference between revisions
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New page: left|200px<br /><applet load="1i6w" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i6w, resolution 1.5Å" /> '''THE CRYSTAL STRUCTURE... |
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[[Image:1i6w.jpg|left|200px]]<br /><applet load="1i6w" size=" | [[Image:1i6w.jpg|left|200px]]<br /><applet load="1i6w" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1i6w, resolution 1.5Å" /> | caption="1i6w, resolution 1.5Å" /> | ||
'''THE CRYSTAL STRUCTURE OF BACILLUS SUBTILIS LIPASE: A MINIMAL ALPHA/BETA HYDROLASE ENZYME'''<br /> | '''THE CRYSTAL STRUCTURE OF BACILLUS SUBTILIS LIPASE: A MINIMAL ALPHA/BETA HYDROLASE ENZYME'''<br /> | ||
==Overview== | ==Overview== | ||
The X-ray structure of the lipase LipA from Bacillus subtilis has been | The X-ray structure of the lipase LipA from Bacillus subtilis has been determined at 1.5 A resolution. It is the first structure of a member of homology family 1.4 of bacterial lipases. The lipase shows a compact minimal alpha/beta hydrolase fold with a six-stranded parallel beta-sheet flanked by five alpha-helices, two on one side of the sheet and three on the other side. The catalytic triad residues, Ser77, Asp133 and His156, and the residues forming the oxyanion hole (backbone amide groups of Ile12 and Met78) are in positions very similar to those of other lipases of known structure. However, no lid domain is present and the active-site nucleophile Ser77 is solvent-exposed. A model of substrate binding is proposed on the basis of a comparison with other lipases with a covalently bound tetrahedral intermediate mimic. It explains the preference of the enzyme for substrates with C8 fatty acid chains. | ||
==About this Structure== | ==About this Structure== | ||
1I6W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with CD as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] Full crystallographic information is available from [http:// | 1I6W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I6W OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Triacylglycerol lipase]] | [[Category: Triacylglycerol lipase]] | ||
[[Category: Dijkstra, B | [[Category: Dijkstra, B W.]] | ||
[[Category: Eggert, T.]] | [[Category: Eggert, T.]] | ||
[[Category: Jaeger, K | [[Category: Jaeger, K E.]] | ||
[[Category: Pouderoyen, G | [[Category: Pouderoyen, G van.]] | ||
[[Category: CD]] | [[Category: CD]] | ||
[[Category: alpha/beta hydrolase]] | [[Category: alpha/beta hydrolase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:08:35 2008'' | ||
