1ir6: Difference between revisions

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[[Image:1ir6.jpg|left|200px]]<br /><applet load="1ir6" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1ir6.jpg|left|200px]]<br /><applet load="1ir6" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1ir6, resolution 2.90Å" />

'''Crystal structure of exonuclease RecJ bound to manganese'''<br />

==Overview==

RecJ, a 5' to 3' exonuclease specific for single-stranded DNA, functions, in DNA repair and recombination systems. We determined the crystal, structure of RecJ bound to Mn(2+) ion essential for its activity. RecJ has, a novel fold in which two domains are interconnected by a long helix, forming a central groove. Mn(2+) is located on the wall of the groove and, is coordinated by conserved residues characteristic of a family of, phosphoesterases that includes RecJ proteins. The groove is composed of, residues conserved among RecJ proteins and is positively charged. These, findings and the narrow width of the groove indicate that the groove binds, single- instead of double-stranded DNA.

RecJ, a 5' to 3' exonuclease specific for single-stranded DNA, functions in DNA repair and recombination systems. We determined the crystal structure of RecJ bound to Mn(2+) ion essential for its activity. RecJ has a novel fold in which two domains are interconnected by a long helix, forming a central groove. Mn(2+) is located on the wall of the groove and is coordinated by conserved residues characteristic of a family of phosphoesterases that includes RecJ proteins. The groove is composed of residues conserved among RecJ proteins and is positively charged. These findings and the narrow width of the groove indicate that the groove binds single- instead of double-stranded DNA.

==About this Structure==

1IR6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with MN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1IR6 OCA].

1IR6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IR6 OCA].

==Reference==

Line 16:

[[Category: Kakuta, Y.]]

[[Category: Masui, R.]]

[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]

[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]

[[Category: Yamagata, A.]]

[[Category: MN]]

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[[Category: two domains interconnected by alpha-helix]]

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@@ Line 1: / Line 1: @@
-[[Image:1ir6.jpg|left|200px]]<br /><applet load="1ir6" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ir6.jpg|left|200px]]<br /><applet load="1ir6" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1ir6, resolution 2.90&Aring;" />
 '''Crystal structure of exonuclease RecJ bound to manganese'''<br />
 ==Overview==
-RecJ, a 5' to 3' exonuclease specific for single-stranded DNA, functions, in DNA repair and recombination systems. We determined the crystal, structure of RecJ bound to Mn(2+) ion essential for its activity. RecJ has, a novel fold in which two domains are interconnected by a long helix, forming a central groove. Mn(2+) is located on the wall of the groove and, is coordinated by conserved residues characteristic of a family of, phosphoesterases that includes RecJ proteins. The groove is composed of, residues conserved among RecJ proteins and is positively charged. These, findings and the narrow width of the groove indicate that the groove binds, single- instead of double-stranded DNA.
+RecJ, a 5' to 3' exonuclease specific for single-stranded DNA, functions in DNA repair and recombination systems. We determined the crystal structure of RecJ bound to Mn(2+) ion essential for its activity. RecJ has a novel fold in which two domains are interconnected by a long helix, forming a central groove. Mn(2+) is located on the wall of the groove and is coordinated by conserved residues characteristic of a family of phosphoesterases that includes RecJ proteins. The groove is composed of residues conserved among RecJ proteins and is positively charged. These findings and the narrow width of the groove indicate that the groove binds single- instead of double-stranded DNA.
 ==About this Structure==
-IR6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with MN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IR6 OCA].
+IR6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IR6 OCA].
 ==Reference==
@@ Line 16: / Line 16: @@
 [[Category: Kakuta, Y.]]
 [[Category: Masui, R.]]
-[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
+[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
 [[Category: Yamagata, A.]]
 [[Category: MN]]
@@ Line 29: / Line 29: @@
 [[Category: two domains interconnected by alpha-helix]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:24:34 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:14:51 2008''