1j5i: Difference between revisions

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[[Image:1j5i.jpg|left|200px]]<br /><applet load="1j5i" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1j5i.jpg|left|200px]]<br /><applet load="1j5i" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1j5i" />

'''Solution Structure of a Novel Chromoprotein Derived from Apo-Neocarzinostatin and a Synthetic Chromophore'''<br />

==Overview==

The natural complex Neocarzinostatin comprises a labile chromophore, noncovalently bound to an 11.2 kDa protein. We present the first, high-resolution structure of a novel complex derived from the recombinant, apoprotein bound to a non-natural synthetic chromophore. Fluorescence and, nuclear magnetic resonance spectroscopy were used to probe the strength, and location of binding. Binding occurred in a location similar to that, observed for the chromophore in the natural Neocarzinostatin complex, but, with a distinct orientation. These results provide structural evidence, that the apoprotein can readily accommodate small druglike entities, other, than the natural chromophore within its binding cleft. The clinical use of, the natural complex described by others, together with the results, reported here, suggests potential applications for small molecule binding, by apo-Neocarzinostatin.

The natural complex Neocarzinostatin comprises a labile chromophore noncovalently bound to an 11.2 kDa protein. We present the first high-resolution structure of a novel complex derived from the recombinant apoprotein bound to a non-natural synthetic chromophore. Fluorescence and nuclear magnetic resonance spectroscopy were used to probe the strength and location of binding. Binding occurred in a location similar to that observed for the chromophore in the natural Neocarzinostatin complex, but with a distinct orientation. These results provide structural evidence that the apoprotein can readily accommodate small druglike entities, other than the natural chromophore within its binding cleft. The clinical use of the natural complex described by others, together with the results reported here, suggests potential applications for small molecule binding by apo-Neocarzinostatin.

==About this Structure==

1J5I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_carzinostaticus Streptomyces carzinostaticus] with NCZ as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1J5I OCA].

1J5I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_carzinostaticus Streptomyces carzinostaticus] with <scene name='pdbligand=NCZ:'>NCZ</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J5I OCA].

==Reference==

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[[Category: ligand]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 17~~:57:~~29 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:19:10 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1j5i.jpg|left|200px]]<br /><applet load="1j5i" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1j5i.jpg|left|200px]]<br /><applet load="1j5i" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1j5i" />
 '''Solution Structure of a Novel Chromoprotein Derived from Apo-Neocarzinostatin and a Synthetic Chromophore'''<br />
 ==Overview==
-The natural complex Neocarzinostatin comprises a labile chromophore, noncovalently bound to an 11.2 kDa protein. We present the first, high-resolution structure of a novel complex derived from the recombinant, apoprotein bound to a non-natural synthetic chromophore. Fluorescence and, nuclear magnetic resonance spectroscopy were used to probe the strength, and location of binding. Binding occurred in a location similar to that, observed for the chromophore in the natural Neocarzinostatin complex, but, with a distinct orientation. These results provide structural evidence, that the apoprotein can readily accommodate small druglike entities, other, than the natural chromophore within its binding cleft. The clinical use of, the natural complex described by others, together with the results, reported here, suggests potential applications for small molecule binding, by apo-Neocarzinostatin.
+The natural complex Neocarzinostatin comprises a labile chromophore noncovalently bound to an 11.2 kDa protein. We present the first high-resolution structure of a novel complex derived from the recombinant apoprotein bound to a non-natural synthetic chromophore. Fluorescence and nuclear magnetic resonance spectroscopy were used to probe the strength and location of binding. Binding occurred in a location similar to that observed for the chromophore in the natural Neocarzinostatin complex, but with a distinct orientation. These results provide structural evidence that the apoprotein can readily accommodate small druglike entities, other than the natural chromophore within its binding cleft. The clinical use of the natural complex described by others, together with the results reported here, suggests potential applications for small molecule binding by apo-Neocarzinostatin.
 ==About this Structure==
-J5I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_carzinostaticus Streptomyces carzinostaticus] with NCZ as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1J5I OCA].
+J5I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_carzinostaticus Streptomyces carzinostaticus] with <scene name='pdbligand=NCZ:'>NCZ</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J5I OCA].
 ==Reference==
@@ Line 19: / Line 19: @@
 [[Category: ligand]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:57:29 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:19:10 2008''