1jes: Difference between revisions

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[[Image:1jes.gif|left|200px]]<br /><applet load="1jes" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1jes.gif|left|200px]]<br /><applet load="1jes" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1jes, resolution 1.5Å" />

'''Crystal Structure of a Copper-Mediated Base Pair in DNA'''<br />

==Overview==

Stable and selective DNA base pairing by metal coordination was recently, demonstrated with nucleotides containing complementary, pyridine-2,6-dicarboxylate (Dipic) and pyridine (Py) bases (Meggers, E.;, Holland, P. L.; Tolman; W. B.; Romesberg, F. E.; Schultz, P. G. J. Am., Chem. Soc. 2000, 122, 10714-10715). To understand the structural, consequences of introducing this novel base pair into DNA we have solved, the crystal structure of a duplex containing the metallo-base pair. The, structure shows that the bases pair as designed, but in a Z-DNA, conformation. The structure also provides a structural explanation for the, B- to Z-DNA transition in this duplex. Further solution studies, demonstrate that the metallo-base pair is compatible with Z- or B-DNA, conformations, depending on the duplex sequence.

Stable and selective DNA base pairing by metal coordination was recently demonstrated with nucleotides containing complementary pyridine-2,6-dicarboxylate (Dipic) and pyridine (Py) bases (Meggers, E.; Holland, P. L.; Tolman; W. B.; Romesberg, F. E.; Schultz, P. G. J. Am. Chem. Soc. 2000, 122, 10714-10715). To understand the structural consequences of introducing this novel base pair into DNA we have solved the crystal structure of a duplex containing the metallo-base pair. The structure shows that the bases pair as designed, but in a Z-DNA conformation. The structure also provides a structural explanation for the B- to Z-DNA transition in this duplex. Further solution studies demonstrate that the metallo-base pair is compatible with Z- or B-DNA conformations, depending on the duplex sequence.

==About this Structure==

1JES is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with CU as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1JES OCA].

1JES is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CU:'>CU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JES OCA].

==Reference==

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[[Category: Atwell, S.]]

[[Category: Meggers, E.]]

[[Category: Schultz, P.G.]]

[[Category: Schultz, P G.]]

[[Category: Spraggon, G.]]

[[Category: CU]]

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[[Category: z-dna]]

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@@ Line 1: / Line 1: @@
-[[Image:1jes.gif|left|200px]]<br /><applet load="1jes" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1jes.gif|left|200px]]<br /><applet load="1jes" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1jes, resolution 1.5&Aring;" />
 '''Crystal Structure of a Copper-Mediated Base Pair in DNA'''<br />
 ==Overview==
-Stable and selective DNA base pairing by metal coordination was recently, demonstrated with nucleotides containing complementary, pyridine-2,6-dicarboxylate (Dipic) and pyridine (Py) bases (Meggers, E.;, Holland, P. L.; Tolman; W. B.; Romesberg, F. E.; Schultz, P. G. J. Am., Chem. Soc. 2000, 122, 10714-10715). To understand the structural, consequences of introducing this novel base pair into DNA we have solved, the crystal structure of a duplex containing the metallo-base pair. The, structure shows that the bases pair as designed, but in a Z-DNA, conformation. The structure also provides a structural explanation for the, B- to Z-DNA transition in this duplex. Further solution studies, demonstrate that the metallo-base pair is compatible with Z- or B-DNA, conformations, depending on the duplex sequence.
+Stable and selective DNA base pairing by metal coordination was recently demonstrated with nucleotides containing complementary pyridine-2,6-dicarboxylate (Dipic) and pyridine (Py) bases (Meggers, E.; Holland, P. L.; Tolman; W. B.; Romesberg, F. E.; Schultz, P. G. J. Am. Chem. Soc. 2000, 122, 10714-10715). To understand the structural consequences of introducing this novel base pair into DNA we have solved the crystal structure of a duplex containing the metallo-base pair. The structure shows that the bases pair as designed, but in a Z-DNA conformation. The structure also provides a structural explanation for the B- to Z-DNA transition in this duplex. Further solution studies demonstrate that the metallo-base pair is compatible with Z- or B-DNA conformations, depending on the duplex sequence.
 ==About this Structure==
-JES is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with CU as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JES OCA].
+JES is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CU:'>CU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JES OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Atwell, S.]]
 [[Category: Meggers, E.]]
-[[Category: Schultz, P.G.]]
+[[Category: Schultz, P G.]]
 [[Category: Spraggon, G.]]
 [[Category: CU]]
@@ Line 27: / Line 27: @@
 [[Category: z-dna]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:32:36 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:21:49 2008''