1jgn: Difference between revisions

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==Overview==

The C-terminal domain of poly(A)-binding protein (PABC) is a, peptide-binding domain found in poly(A)-binding proteins (PABPs) and a, HECT (homologous to E6-AP C-terminus) family E3 ubiquitin ligase. In, protein synthesis, the PABC domain of PABP functions to recruit several, translation factors possessing the PABP-interacting motif 2 (PAM2) to the, mRNA poly(A) tail. We have determined the solution structure of the human, PABC domain in complex with two peptides from PABP-interacting protein-1, (Paip1) and Paip2. The structures show a novel mode of peptide, recognition, in which the peptide binds as a pair of beta-turns with, extensive hydrophobic, electrostatic and aromatic stacking interactions., Mutagenesis of PABC and peptide residues was used to identify key, protein-peptide interactions and quantified by isothermal calorimetry, surface plasmon resonance and GST pull-down assays. The results provide, insight into the specificity of PABC in mediating PABP-protein, interactions.

The C-terminal domain of poly(A)-binding protein (PABC) is a peptide-binding domain found in poly(A)-binding proteins (PABPs) and a HECT (homologous to E6-AP C-terminus) family E3 ubiquitin ligase. In protein synthesis, the PABC domain of PABP functions to recruit several translation factors possessing the PABP-interacting motif 2 (PAM2) to the mRNA poly(A) tail. We have determined the solution structure of the human PABC domain in complex with two peptides from PABP-interacting protein-1 (Paip1) and Paip2. The structures show a novel mode of peptide recognition, in which the peptide binds as a pair of beta-turns with extensive hydrophobic, electrostatic and aromatic stacking interactions. Mutagenesis of PABC and peptide residues was used to identify key protein-peptide interactions and quantified by isothermal calorimetry, surface plasmon resonance and GST pull-down assays. The results provide insight into the specificity of PABC in mediating PABP-protein interactions.

==About this Structure==

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[[Category: protein-peptide complex]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-The C-terminal domain of poly(A)-binding protein (PABC) is a, peptide-binding domain found in poly(A)-binding proteins (PABPs) and a, HECT (homologous to E6-AP C-terminus) family E3 ubiquitin ligase. In, protein synthesis, the PABC domain of PABP functions to recruit several, translation factors possessing the PABP-interacting motif 2 (PAM2) to the, mRNA poly(A) tail. We have determined the solution structure of the human, PABC domain in complex with two peptides from PABP-interacting protein-1, (Paip1) and Paip2. The structures show a novel mode of peptide, recognition, in which the peptide binds as a pair of beta-turns with, extensive hydrophobic, electrostatic and aromatic stacking interactions., Mutagenesis of PABC and peptide residues was used to identify key, protein-peptide interactions and quantified by isothermal calorimetry, surface plasmon resonance and GST pull-down assays. The results provide, insight into the specificity of PABC in mediating PABP-protein, interactions.
+The C-terminal domain of poly(A)-binding protein (PABC) is a peptide-binding domain found in poly(A)-binding proteins (PABPs) and a HECT (homologous to E6-AP C-terminus) family E3 ubiquitin ligase. In protein synthesis, the PABC domain of PABP functions to recruit several translation factors possessing the PABP-interacting motif 2 (PAM2) to the mRNA poly(A) tail. We have determined the solution structure of the human PABC domain in complex with two peptides from PABP-interacting protein-1 (Paip1) and Paip2. The structures show a novel mode of peptide recognition, in which the peptide binds as a pair of beta-turns with extensive hydrophobic, electrostatic and aromatic stacking interactions. Mutagenesis of PABC and peptide residues was used to identify key protein-peptide interactions and quantified by isothermal calorimetry, surface plasmon resonance and GST pull-down assays. The results provide insight into the specificity of PABC in mediating PABP-protein interactions.
 ==About this Structure==
@@ Line 21: / Line 21: @@
 [[Category: protein-peptide complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:07:15 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:22:25 2008''