1jm1: Difference between revisions

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[[Image:1jm1.jpg|left|200px]]<br /><applet load="1jm1" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1jm1.jpg|left|200px]]<br /><applet load="1jm1" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1jm1, resolution 1.11Å" />

'''Crystal structure of the soluble domain of the Rieske protein II (soxF) from Sulfolobus acidocaldarius'''<br />

==Overview==

The first crystal structure of an archaeal Rieske iron-sulfur protein, the, soluble domain of Rieske iron-sulfur protein II (soxF) from the, hyperthermo-acidophile Sulfolobus acidocaldarius, has been solved by, multiple wavelength anomalous dispersion (MAD) and has been refined to 1.1, A resolution. SoxF is a subunit of the terminal oxidase supercomplex SoxM, in the plasma membrane of S. acidocaldarius that combines features of a, cytochrome bc(1) complex and a cytochrome c oxidase. The [2Fe-2S] cluster, of soxF is most likely the primary electron acceptor during the oxidation, of caldariella quinone by the cytochrome a(587)/Rieske subcomplex. The, geometry of the [2Fe-2S] cluster and the structure of the cluster-binding, site are almost identical in soxF and the Rieske proteins from eucaryal, cytochrome bc(1) and b(6)f complexes, suggesting a strict conservation of, the catalytic mechanism. The main domain of soxF and part of the, cluster-binding domain, though structurally related, show a significantly, divergent structure with respect to topology, non-covalent interactions, and surface charges. The divergent structure of soxF reflects a different, topology of the soxM complex compared to eucaryal bc complexes and the, adaptation of the protein to the extreme ambient conditions on the outer, membrane surface of a hyperthermo-acidophilic organism.

The first crystal structure of an archaeal Rieske iron-sulfur protein, the soluble domain of Rieske iron-sulfur protein II (soxF) from the hyperthermo-acidophile Sulfolobus acidocaldarius, has been solved by multiple wavelength anomalous dispersion (MAD) and has been refined to 1.1 A resolution. SoxF is a subunit of the terminal oxidase supercomplex SoxM in the plasma membrane of S. acidocaldarius that combines features of a cytochrome bc(1) complex and a cytochrome c oxidase. The [2Fe-2S] cluster of soxF is most likely the primary electron acceptor during the oxidation of caldariella quinone by the cytochrome a(587)/Rieske subcomplex. The geometry of the [2Fe-2S] cluster and the structure of the cluster-binding site are almost identical in soxF and the Rieske proteins from eucaryal cytochrome bc(1) and b(6)f complexes, suggesting a strict conservation of the catalytic mechanism. The main domain of soxF and part of the cluster-binding domain, though structurally related, show a significantly divergent structure with respect to topology, non-covalent interactions and surface charges. The divergent structure of soxF reflects a different topology of the soxM complex compared to eucaryal bc complexes and the adaptation of the protein to the extreme ambient conditions on the outer membrane surface of a hyperthermo-acidophilic organism.

==About this Structure==

1JM1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius] with MG and FES as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1JM1 OCA].

1JM1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=FES:'>FES</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JM1 OCA].

==Reference==

Line 16:

[[Category: Ladenstein, R.]]

[[Category: Schaefer, G.]]

[[Category: Schmidt, C.L.]]

[[Category: Schmidt, C L.]]

[[Category: FES]]

[[Category: MG]]

Line 23:

[[Category: rieske iron-sulfur protein]]

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@@ Line 1: / Line 1: @@
-[[Image:1jm1.jpg|left|200px]]<br /><applet load="1jm1" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1jm1.jpg|left|200px]]<br /><applet load="1jm1" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1jm1, resolution 1.11&Aring;" />
 '''Crystal structure of the soluble domain of the Rieske protein II (soxF) from Sulfolobus acidocaldarius'''<br />
 ==Overview==
-The first crystal structure of an archaeal Rieske iron-sulfur protein, the, soluble domain of Rieske iron-sulfur protein II (soxF) from the, hyperthermo-acidophile Sulfolobus acidocaldarius, has been solved by, multiple wavelength anomalous dispersion (MAD) and has been refined to 1.1, A resolution. SoxF is a subunit of the terminal oxidase supercomplex SoxM, in the plasma membrane of S. acidocaldarius that combines features of a, cytochrome bc(1) complex and a cytochrome c oxidase. The [2Fe-2S] cluster, of soxF is most likely the primary electron acceptor during the oxidation, of caldariella quinone by the cytochrome a(587)/Rieske subcomplex. The, geometry of the [2Fe-2S] cluster and the structure of the cluster-binding, site are almost identical in soxF and the Rieske proteins from eucaryal, cytochrome bc(1) and b(6)f complexes, suggesting a strict conservation of, the catalytic mechanism. The main domain of soxF and part of the, cluster-binding domain, though structurally related, show a significantly, divergent structure with respect to topology, non-covalent interactions, and surface charges. The divergent structure of soxF reflects a different, topology of the soxM complex compared to eucaryal bc complexes and the, adaptation of the protein to the extreme ambient conditions on the outer, membrane surface of a hyperthermo-acidophilic organism.
+The first crystal structure of an archaeal Rieske iron-sulfur protein, the soluble domain of Rieske iron-sulfur protein II (soxF) from the hyperthermo-acidophile Sulfolobus acidocaldarius, has been solved by multiple wavelength anomalous dispersion (MAD) and has been refined to 1.1 A resolution. SoxF is a subunit of the terminal oxidase supercomplex SoxM in the plasma membrane of S. acidocaldarius that combines features of a cytochrome bc(1) complex and a cytochrome c oxidase. The [2Fe-2S] cluster of soxF is most likely the primary electron acceptor during the oxidation of caldariella quinone by the cytochrome a(587)/Rieske subcomplex. The geometry of the [2Fe-2S] cluster and the structure of the cluster-binding site are almost identical in soxF and the Rieske proteins from eucaryal cytochrome bc(1) and b(6)f complexes, suggesting a strict conservation of the catalytic mechanism. The main domain of soxF and part of the cluster-binding domain, though structurally related, show a significantly divergent structure with respect to topology, non-covalent interactions and surface charges. The divergent structure of soxF reflects a different topology of the soxM complex compared to eucaryal bc complexes and the adaptation of the protein to the extreme ambient conditions on the outer membrane surface of a hyperthermo-acidophilic organism.
 ==About this Structure==
-JM1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius] with MG and FES as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JM1 OCA].
+JM1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=FES:'>FES</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JM1 OCA].
 ==Reference==
@@ Line 16: / Line 16: @@
 [[Category: Ladenstein, R.]]
 [[Category: Schaefer, G.]]
-[[Category: Schmidt, C.L.]]
+[[Category: Schmidt, C L.]]
 [[Category: FES]]
 [[Category: MG]]
@@ Line 23: / Line 23: @@
 [[Category: rieske iron-sulfur protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:51:51 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:24:14 2008''