1jmt: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
U2 auxiliary factor (U2AF) is an essential splicing factor that recognizes | U2 auxiliary factor (U2AF) is an essential splicing factor that recognizes the 3' splice site and recruits the U2 snRNP to the branch point. The X-ray structure of the human core U2AF heterodimer, consisting of the U2AF35 central domain and a proline-rich region of U2AF65, has been determined at 2.2 A resolution. The structure reveals a novel protein-protein recognition strategy, in which an atypical RNA recognition motif (RRM) of U2AF35 and the U2AF65 polyproline segment interact via reciprocal "tongue-in-groove" tryptophan residues. Complementary biochemical experiments demonstrate that the core U2AF heterodimer binds RNA, and that the interacting tryptophan side chains are essential for U2AF dimerization. Atypical RRMs in other splicing factors may serve as protein-protein interaction motifs elsewhere during spliceosome assembly. | ||
==About this Structure== | ==About this Structure== | ||
| Line 13: | Line 13: | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Burley, S | [[Category: Burley, S K.]] | ||
[[Category: Green, M | [[Category: Green, M R.]] | ||
[[Category: Kielkopf, C | [[Category: Kielkopf, C L.]] | ||
[[Category: Rodionova, N | [[Category: Rodionova, N A.]] | ||
[[Category: HEZ]] | [[Category: HEZ]] | ||
[[Category: peptide recognition]] | [[Category: peptide recognition]] | ||
| Line 24: | Line 24: | ||
[[Category: rrm]] | [[Category: rrm]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:24:22 2008'' | ||
