CcNiR: Difference between revisions

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== Your Heading Here ('ccNiR') ==
== Your Heading Here ('ccNiR') ==
<StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''>
<StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''>
Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme (see <scene name='CcNiR/Fe/4'>iron</scene>) to perform the reduction of nitrite to ammonium  in a six-electron transfer reaction <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 20689707</ref>
Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme (see <scene name='CcNiR/Fe/2'>iron</scene>) to perform the reduction of nitrite to ammonium  in a six-electron transfer reaction <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 20689707</ref>





Revision as of 09:38, 6 September 2012

Your Heading Here ('ccNiR')

CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry 1oah)

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Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.

The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).


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