1jvo: Difference between revisions

Line 1:

[[Image:1jvo.gif|left|200px]]<br /><applet load="1jvo" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1jvo.gif|left|200px]]<br /><applet load="1jvo" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1jvo, resolution 2.75Å" />

'''Azurin dimer, crosslinked via disulfide bridge'''<br />

==Overview==

The transfer of electrons between proteins is an essential step in, biological energy production. Two protein redox partners are often, artificially crosslinked to investigate the poorly understood mechanism by, which they interact. To better understand the effect of crosslinking on, electron transfer rates, we have constructed dimers of azurin by, crosslinking the monomers. The measured electron exchange rates, combined, with crystal structures of the dimers, demonstrate that the length of the, linker can have a dramatic effect on the structure of the dimer and the, electron transfer rate. The presence of ordered water molecules in the, protein-protein interface may considerably influence the electronic, coupling between redox centers.

The transfer of electrons between proteins is an essential step in biological energy production. Two protein redox partners are often artificially crosslinked to investigate the poorly understood mechanism by which they interact. To better understand the effect of crosslinking on electron transfer rates, we have constructed dimers of azurin by crosslinking the monomers. The measured electron exchange rates, combined with crystal structures of the dimers, demonstrate that the length of the linker can have a dramatic effect on the structure of the dimer and the electron transfer rate. The presence of ordered water molecules in the protein-protein interface may considerably influence the electronic coupling between redox centers.

==About this Structure==

1JVO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with CU as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1JVO OCA].

1JVO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=CU:'>CU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JVO OCA].

==Reference==

Line 13:

[[Category: Pseudomonas aeruginosa]]

[[Category: Single protein]]

[[Category: Amsterdam, I.M.C.van.]]

[[Category: Amsterdam, I M.C van.]]

[[Category: Canters, G.W.]]

[[Category: Canters, G W.]]

[[Category: Cavazzini, D.]]

[[Category: Einsle, O.]]

[[Category: Merli, A.]]

[[Category: Messerschmidt, A.]]

[[Category: Rossi, G.L.]]

[[Category: Rossi, G L.]]

[[Category: Ubbink, M.]]

[[Category: CU]]

Line 26:

[[Category: electron transfer]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 18~~:36:~~51 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:27:14 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1jvo.gif|left|200px]]<br /><applet load="1jvo" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1jvo.gif|left|200px]]<br /><applet load="1jvo" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1jvo, resolution 2.75&Aring;" />
 '''Azurin dimer, crosslinked via disulfide bridge'''<br />
 ==Overview==
-The transfer of electrons between proteins is an essential step in, biological energy production. Two protein redox partners are often, artificially crosslinked to investigate the poorly understood mechanism by, which they interact. To better understand the effect of crosslinking on, electron transfer rates, we have constructed dimers of azurin by, crosslinking the monomers. The measured electron exchange rates, combined, with crystal structures of the dimers, demonstrate that the length of the, linker can have a dramatic effect on the structure of the dimer and the, electron transfer rate. The presence of ordered water molecules in the, protein-protein interface may considerably influence the electronic, coupling between redox centers.
+The transfer of electrons between proteins is an essential step in biological energy production. Two protein redox partners are often artificially crosslinked to investigate the poorly understood mechanism by which they interact. To better understand the effect of crosslinking on electron transfer rates, we have constructed dimers of azurin by crosslinking the monomers. The measured electron exchange rates, combined with crystal structures of the dimers, demonstrate that the length of the linker can have a dramatic effect on the structure of the dimer and the electron transfer rate. The presence of ordered water molecules in the protein-protein interface may considerably influence the electronic coupling between redox centers.
 ==About this Structure==
-JVO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with CU as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JVO OCA].
+JVO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=CU:'>CU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JVO OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Pseudomonas aeruginosa]]
 [[Category: Single protein]]
-[[Category: Amsterdam, I.M.C.van.]]
+[[Category: Amsterdam, I M.C van.]]
-[[Category: Canters, G.W.]]
+[[Category: Canters, G W.]]
 [[Category: Cavazzini, D.]]
 [[Category: Einsle, O.]]
 [[Category: Merli, A.]]
 [[Category: Messerschmidt, A.]]
-[[Category: Rossi, G.L.]]
+[[Category: Rossi, G L.]]
 [[Category: Ubbink, M.]]
 [[Category: CU]]
@@ Line 26: / Line 26: @@
 [[Category: electron transfer]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:36:51 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:27:14 2008''