Introduction to Evolutionary Conservation: Difference between revisions
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===Locations of Mutations in 3D Model=== | ===Locations of Mutations in 3D Model=== | ||
<Structure load='' size='350' frame='true' align='right' caption='MeCp2 protein bound to DNA (crystal structure [[3cpi]]).' scene='Introduction_to_Evolutionary_Conservation/Conservation/1' /> | <Structure load='' size='350' frame='true' align='right' caption='MeCp2 protein bound to DNA (crystal structure [[3cpi]]). Conservation calculated by [[ConSurfDB_vs._ConSurf|ConSurf-DB]].' scene='Introduction_to_Evolutionary_Conservation/Conservation/1' /> | ||
The positions of <font color='#c000a8'>'''conserved Arg133'''</font> and <font color='00a0a0'>'''variable Glu143'''</font> are highlighted with <span style="background:black; color:yellow;">''' yellow '''</span> halos. You can see that <font color='#c000a8'>'''conserved Arg133'''</font> is in intimate contact with the <font color='#d46a42'>'''DNA'''</font>, while <font color='00a0a0'>'''variable Glu143'''</font> is on the surface, and remote from the contact with the <font color='#d46a42'>'''DNA'''</font>. | The positions of <font color='#c000a8'>'''conserved Arg133'''</font> and <font color='00a0a0'>'''variable Glu143'''</font> are highlighted with <span style="background:black; color:yellow;">''' yellow '''</span> halos. You can see that <font color='#c000a8'>'''conserved Arg133'''</font> is in intimate contact with the <font color='#d46a42'>'''DNA'''</font>, while <font color='00a0a0'>'''variable Glu143'''</font> is on the surface, and remote from the contact with the <font color='#d46a42'>'''DNA'''</font>. | ||
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{{Clear}} | {{Clear}} | ||
==Finding Conservation== | ==Finding Conservation== | ||
Evolutionary conservation in proteins is identified by aligning the amino acid sequences of proteins with the same function from different taxa (orthologs). As an example, we'll use the glycolytic enzyme '''enolase''', present in a wide range of taxa. Take a quick look to get an impression of a [[Enolase multiple sequence alignment|multiple sequence alignment for ~400 amino acids in enolase]] for taxa ranging from eubacteria and archaebacteria through yeast, insects, and humans. | Evolutionary conservation in proteins is identified by aligning the amino acid sequences of proteins with the same function from different taxa (orthologs). As an example, we'll use the glycolytic enzyme '''enolase''', present in a wide range of taxa. Take a quick look to get an impression of a [[Enolase multiple sequence alignment|multiple sequence alignment for ~400 amino acids in enolase]] for taxa ranging from eubacteria and archaebacteria through yeast, insects, and humans. | ||
