1kaq: Difference between revisions
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New page: left|200px<br /><applet load="1kaq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kaq, resolution 3.20Å" /> '''Structure of Bacillu... |
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[[Image:1kaq.gif|left|200px]]<br /><applet load="1kaq" size=" | [[Image:1kaq.gif|left|200px]]<br /><applet load="1kaq" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1kaq, resolution 3.20Å" /> | caption="1kaq, resolution 3.20Å" /> | ||
'''Structure of Bacillus subtilis Nicotinic Acid Mononucleotide Adenylyl Transferase'''<br /> | '''Structure of Bacillus subtilis Nicotinic Acid Mononucleotide Adenylyl Transferase'''<br /> | ||
==Overview== | ==Overview== | ||
The nadD gene, encoding the enzyme nicotinic acid mononucleotide (NaMN) | The nadD gene, encoding the enzyme nicotinic acid mononucleotide (NaMN) adenylyltransferase (AT), is essential for the synthesis of NAD and subsequent viability of the cell. The nadD gene in Bacillus subtilis (yqeJ) was identified by sequence homology with other bacterial nadD genes and by biochemical characterization of the gene product. NaMN AT catalyzes the reversible adenylation of both NaMN and the nicotinamide mononucleotide (NMN) but shows specificity for the nicotinate. In contrast to other known NMN ATs, biophysical characterizations reveal it to be a dimer. The NaMN AT crystal structure was determined for both the apo enzyme and product-bound form, to 2.1 and 3.2 A, respectively. The structures reveal a "functional" dimer conserved in both crystal forms and a monomer fold common to members of the nucleotidyl-transferase alpha/beta phosphodiesterase superfamily. A structural comparison with family members suggests a new conserved motif (SXXXX(R/K)) at the N terminus of an alpha-helix, which is not part of the shared fold. Interactions of the nicotinic acid with backbone atoms indicate the structural basis for specificity. | ||
==About this Structure== | ==About this Structure== | ||
1KAQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with DND as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Nicotinate-nucleotide_adenylyltransferase Nicotinate-nucleotide adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.18 2.7.7.18] Full crystallographic information is available from [http:// | 1KAQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=DND:'>DND</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Nicotinate-nucleotide_adenylyltransferase Nicotinate-nucleotide adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.18 2.7.7.18] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KAQ OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Bard, J.]] | [[Category: Bard, J.]] | ||
[[Category: Chopra, R.]] | [[Category: Chopra, R.]] | ||
[[Category: Czerwinski, R | [[Category: Czerwinski, R M.]] | ||
[[Category: Lo, M | [[Category: Lo, M C.]] | ||
[[Category: Olland, A | [[Category: Olland, A M.]] | ||
[[Category: Somers, W | [[Category: Somers, W S.]] | ||
[[Category: Stahl, M | [[Category: Stahl, M L.]] | ||
[[Category: Sullivan, F | [[Category: Sullivan, F X.]] | ||
[[Category: Underwood, K | [[Category: Underwood, K W.]] | ||
[[Category: DND]] | [[Category: DND]] | ||
[[Category: naad]] | [[Category: naad]] | ||
[[Category: rossman fold]] | [[Category: rossman fold]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:31:58 2008'' | ||
