1kaw: Difference between revisions
New page: left|200px<br /><applet load="1kaw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kaw, resolution 2.9Å" /> '''STRUCTURE OF SINGLE S... |
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[[Image:1kaw.jpg|left|200px]]<br /><applet load="1kaw" size=" | [[Image:1kaw.jpg|left|200px]]<br /><applet load="1kaw" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1kaw, resolution 2.9Å" /> | caption="1kaw, resolution 2.9Å" /> | ||
'''STRUCTURE OF SINGLE STRANDED DNA BINDING PROTEIN (SSB)'''<br /> | '''STRUCTURE OF SINGLE STRANDED DNA BINDING PROTEIN (SSB)'''<br /> | ||
==Overview== | ==Overview== | ||
The crystal structure of the tetrameric DNA-binding domain of the | The crystal structure of the tetrameric DNA-binding domain of the single-stranded DNA binding protein from Escherichia coli was determined at a resolution of 2.9 A using multiwavelength anomalous dispersion. Each monomer in the tetramer is topologically similar to an oligomer-binding fold. Two monomers each contribute three beta-strands to a single six-stranded beta-sheet to form a dimer. Two dimer-dimer interfaces are observed within the crystal. One of these stabilizes the tetramer in solution. The other interface promotes a superhelical structure within the crystal that may reflect tetramer-tetramer interactions involved in the positive cooperative binding of the single-stranded DNA-binding protein to single-stranded DNA. | ||
==About this Structure== | ==About this Structure== | ||
1KAW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | 1KAW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KAW OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: ssb]] | [[Category: ssb]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:32:00 2008'' | ||
Revision as of 11:32, 21 February 2008
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STRUCTURE OF SINGLE STRANDED DNA BINDING PROTEIN (SSB)
Overview
The crystal structure of the tetrameric DNA-binding domain of the single-stranded DNA binding protein from Escherichia coli was determined at a resolution of 2.9 A using multiwavelength anomalous dispersion. Each monomer in the tetramer is topologically similar to an oligomer-binding fold. Two monomers each contribute three beta-strands to a single six-stranded beta-sheet to form a dimer. Two dimer-dimer interfaces are observed within the crystal. One of these stabilizes the tetramer in solution. The other interface promotes a superhelical structure within the crystal that may reflect tetramer-tetramer interactions involved in the positive cooperative binding of the single-stranded DNA-binding protein to single-stranded DNA.
About this Structure
1KAW is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of the homo-tetrameric DNA binding domain of Escherichia coli single-stranded DNA-binding protein determined by multiwavelength x-ray diffraction on the selenomethionyl protein at 2.9-A resolution., Raghunathan S, Ricard CS, Lohman TM, Waksman G, Proc Natl Acad Sci U S A. 1997 Jun 24;94(13):6652-7. PMID:9192620
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