1l0a: Difference between revisions

Line 4:

==Overview==

TRAFs (tumor necrosis factor receptor [TNFR]-associated factors) bind to, the cytoplasmic portion of liganded TNFRs and stimulate activation of, NF-kappaB or JNK pathways. A modulator of TRAF signaling, TANK, serves as, either an enhancer or an inhibitor of TRAF-mediated signaling pathways., The crystal structure of a region of TANK bound to TRAF3 has been, determined and compared to a similar CD40/TRAF3 complex. TANK and CD40, bind to the same crevice on TRAF3. The recognition motif PxQxT is, presented in a boomerang-like structure in TANK that is markedly different, from the hairpin loop that forms in CD40 upon binding to TRAF3. Critical, TANK contact residues were confirmed by mutagenesis to be required for, binding to TRAF3 or TRAF2. Binding affinity, measured by isothermal, titration calorimetry and competition assays, demonstrated that TANK, competes with CD40 for the TRAF binding site.

TRAFs (tumor necrosis factor receptor [TNFR]-associated factors) bind to the cytoplasmic portion of liganded TNFRs and stimulate activation of NF-kappaB or JNK pathways. A modulator of TRAF signaling, TANK, serves as either an enhancer or an inhibitor of TRAF-mediated signaling pathways. The crystal structure of a region of TANK bound to TRAF3 has been determined and compared to a similar CD40/TRAF3 complex. TANK and CD40 bind to the same crevice on TRAF3. The recognition motif PxQxT is presented in a boomerang-like structure in TANK that is markedly different from the hairpin loop that forms in CD40 upon binding to TRAF3. Critical TANK contact residues were confirmed by mutagenesis to be required for binding to TRAF3 or TRAF2. Binding affinity, measured by isothermal titration calorimetry and competition assays, demonstrated that TANK competes with CD40 for the TRAF binding site.

==About this Structure==

Line 15:

[[Category: Cabezas, E.]]

[[Category: Cheng, G.]]

[[Category: Ely, K.R.]]

[[Category: Ely, K R.]]

[[Category: Havert, M.L.]]

[[Category: Havert, M L.]]

[[Category: He, J.]]

[[Category: Kaiser, D.]]

[[Category: Li, C.]]

[[Category: Ni, C.Z.]]

[[Category: Ni, C Z.]]

[[Category: Reed, J.C.]]

[[Category: Reed, J C.]]

[[Category: Satterthwait, A.C.]]

[[Category: Satterthwait, A C.]]

[[Category: cd40]]

[[Category: nf-kb signaling]]

Line 29:

[[Category: traf3]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri~~ Feb ~~15 16~~:16:07 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:39:53 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 4: / Line 4: @@
 ==Overview==
-TRAFs (tumor necrosis factor receptor [TNFR]-associated factors) bind to, the cytoplasmic portion of liganded TNFRs and stimulate activation of, NF-kappaB or JNK pathways. A modulator of TRAF signaling, TANK, serves as, either an enhancer or an inhibitor of TRAF-mediated signaling pathways., The crystal structure of a region of TANK bound to TRAF3 has been, determined and compared to a similar CD40/TRAF3 complex. TANK and CD40, bind to the same crevice on TRAF3. The recognition motif PxQxT is, presented in a boomerang-like structure in TANK that is markedly different, from the hairpin loop that forms in CD40 upon binding to TRAF3. Critical, TANK contact residues were confirmed by mutagenesis to be required for, binding to TRAF3 or TRAF2. Binding affinity, measured by isothermal, titration calorimetry and competition assays, demonstrated that TANK, competes with CD40 for the TRAF binding site.
+TRAFs (tumor necrosis factor receptor [TNFR]-associated factors) bind to the cytoplasmic portion of liganded TNFRs and stimulate activation of NF-kappaB or JNK pathways. A modulator of TRAF signaling, TANK, serves as either an enhancer or an inhibitor of TRAF-mediated signaling pathways. The crystal structure of a region of TANK bound to TRAF3 has been determined and compared to a similar CD40/TRAF3 complex. TANK and CD40 bind to the same crevice on TRAF3. The recognition motif PxQxT is presented in a boomerang-like structure in TANK that is markedly different from the hairpin loop that forms in CD40 upon binding to TRAF3. Critical TANK contact residues were confirmed by mutagenesis to be required for binding to TRAF3 or TRAF2. Binding affinity, measured by isothermal titration calorimetry and competition assays, demonstrated that TANK competes with CD40 for the TRAF binding site.
 ==About this Structure==
@@ Line 15: / Line 15: @@
 [[Category: Cabezas, E.]]
 [[Category: Cheng, G.]]
-[[Category: Ely, K.R.]]
+[[Category: Ely, K R.]]
-[[Category: Havert, M.L.]]
+[[Category: Havert, M L.]]
 [[Category: He, J.]]
 [[Category: Kaiser, D.]]
 [[Category: Li, C.]]
-[[Category: Ni, C.Z.]]
+[[Category: Ni, C Z.]]
-[[Category: Reed, J.C.]]
+[[Category: Reed, J C.]]
-[[Category: Satterthwait, A.C.]]
+[[Category: Satterthwait, A C.]]
 [[Category: cd40]]
 [[Category: nf-kb signaling]]
@@ Line 29: / Line 29: @@
 [[Category: traf3]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:16:07 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:39:53 2008''