1l2h: Difference between revisions
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==Overview== | ==Overview== | ||
The crystal structure at 1.54 A resolution of a double mutant of | The crystal structure at 1.54 A resolution of a double mutant of interleukin-1beta (F42W/W120F), a cytokine secreted by macrophages, was determined by multiple-wavelength anomalous dispersion (MAD) using data from highly twinned selenomethionine-modified crystals. The space group is P4(3), with unit-cell parameters a = b = 53.9, c = 77.4 A. Self-rotation function analysis and various intensity statistics revealed the presence of merohedral twinning in crystals of both the native (twinning fraction alpha approximately 0.35) and SeMet (alpha approximately 0.40) forms. Structure determination and refinement are discussed with emphasis on the possible reasons for successful phasing using untreated twinned MAD data. | ||
==Disease== | ==Disease== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Heidary, D | [[Category: Heidary, D K.]] | ||
[[Category: Jennings, P | [[Category: Jennings, P A.]] | ||
[[Category: Kelker, M | [[Category: Kelker, M S.]] | ||
[[Category: Oseroff, V.]] | [[Category: Oseroff, V.]] | ||
[[Category: Rudolph, M | [[Category: Rudolph, M G.]] | ||
[[Category: Schneider, T | [[Category: Schneider, T R.]] | ||
[[Category: Wilson, I | [[Category: Wilson, I A.]] | ||
[[Category: Yeates, T | [[Category: Yeates, T O.]] | ||
[[Category: beta-trefoil]] | [[Category: beta-trefoil]] | ||
[[Category: f42w/w120f double mutant]] | [[Category: f42w/w120f double mutant]] | ||
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[[Category: protein folding]] | [[Category: protein folding]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:40:34 2008'' | ||
