1lc6: Difference between revisions
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'''Solution Structure of the U6 Intramolecular Stem-loop RNA'''<br /> | '''Solution Structure of the U6 Intramolecular Stem-loop RNA'''<br /> | ||
==Overview== | ==Overview== | ||
U6 RNA is a key component of the catalytic core of the spliceosome. A | U6 RNA is a key component of the catalytic core of the spliceosome. A metal ion essential for the first catalytic step of pre-mRNA splicing binds to the U80 Sp phosphate oxygen within the yeast U6 intramolecular stem-loop (ISL). Here we present the first structural data for U6 RNA, revealing the three-dimensional structure of the highly conserved U6 ISL. The ISL binds metal ion at the U80 site with the same stereo specificity as the intact spliceosome. The metal-binding site is adjacent to a readily protonated C.A wobble pair. Protonation of the C.A pair and metal binding are mutually antagonistic. These results support a ribozyme model for U6 RNA function and suggest a possible mechanism for the regulation of RNA splicing. | ||
==About this Structure== | ==About this Structure== | ||
1LC6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | 1LC6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LC6 OCA]. | ||
==Reference== | ==Reference== | ||
Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure., Huppler A, Nikstad LJ, Allmann AM, Brow DA, Butcher SE, Nat Struct Biol. 2002 Jun;9(6):431-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11992125 11992125] | Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure., Huppler A, Nikstad LJ, Allmann AM, Brow DA, Butcher SE, Nat Struct Biol. 2002 Jun;9(6):431-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11992125 11992125] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Allmann, A | [[Category: Allmann, A M.]] | ||
[[Category: Brow, D | [[Category: Brow, D A.]] | ||
[[Category: Butcher, S | [[Category: Butcher, S E.]] | ||
[[Category: Huppler, A.]] | [[Category: Huppler, A.]] | ||
[[Category: Nikstad, L | [[Category: Nikstad, L J.]] | ||
[[Category: pentaloop]] | [[Category: pentaloop]] | ||
[[Category: rna]] | [[Category: rna]] | ||
[[Category: stem-loop]] | [[Category: stem-loop]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:43:35 2008'' | ||
Revision as of 11:43, 21 February 2008
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Solution Structure of the U6 Intramolecular Stem-loop RNA
Overview
U6 RNA is a key component of the catalytic core of the spliceosome. A metal ion essential for the first catalytic step of pre-mRNA splicing binds to the U80 Sp phosphate oxygen within the yeast U6 intramolecular stem-loop (ISL). Here we present the first structural data for U6 RNA, revealing the three-dimensional structure of the highly conserved U6 ISL. The ISL binds metal ion at the U80 site with the same stereo specificity as the intact spliceosome. The metal-binding site is adjacent to a readily protonated C.A wobble pair. Protonation of the C.A pair and metal binding are mutually antagonistic. These results support a ribozyme model for U6 RNA function and suggest a possible mechanism for the regulation of RNA splicing.
About this Structure
1LC6 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure., Huppler A, Nikstad LJ, Allmann AM, Brow DA, Butcher SE, Nat Struct Biol. 2002 Jun;9(6):431-5. PMID:11992125
Page seeded by OCA on Thu Feb 21 13:43:35 2008
