1lf8: Difference between revisions

Line 1:

[[Image:1lf8.gif|left|200px]]<br />

[[Image:1lf8.gif|left|200px]]<br /><applet load="1lf8" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1lf8" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1lf8, resolution 2.30Å" />

'''Complex of GGA3-VHS Domain and CI-MPR C-terminal Phosphopeptide'''<br />

==Overview==

Phosphorylation of the cytosolic tails of transmembrane receptors can, regulate their intracellular trafficking. The structural basis for such, regulation, however, has not been explained in most cases. The cytosolic, tail of the cation-independent mannose 6-phosphate receptor contains a, serine residue within an acidic-cluster dileucine signal that is important, for the function of the receptor in the biosynthetic sorting of lysosomal, hydrolases. We show here that phosphorylation of this Ser enhances, interactions of the signal with its recognition module, the VHS domain of, the GGA proteins. Crystallographic analyses demonstrate that the, phosphoserine residue interacts electrostatically with two basic residues, on the VHS domain of GGA3, thus providing an additional point of, attachment of the acidic-cluster dileucine signal to its recognition, module.

Phosphorylation of the cytosolic tails of transmembrane receptors can regulate their intracellular trafficking. The structural basis for such regulation, however, has not been explained in most cases. The cytosolic tail of the cation-independent mannose 6-phosphate receptor contains a serine residue within an acidic-cluster dileucine signal that is important for the function of the receptor in the biosynthetic sorting of lysosomal hydrolases. We show here that phosphorylation of this Ser enhances interactions of the signal with its recognition module, the VHS domain of the GGA proteins. Crystallographic analyses demonstrate that the phosphoserine residue interacts electrostatically with two basic residues on the VHS domain of GGA3, thus providing an additional point of attachment of the acidic-cluster dileucine signal to its recognition module.

==Disease==

Line 11:

Line 10:

==About this Structure==

1LF8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1LF8 OCA].

1LF8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LF8 OCA].

==Reference==

Line 17:

Line 16:

[[Category: Homo sapiens]]

[[Category: Protein complex]]

[[Category: Bonifacino, J.S.]]

[[Category: Bonifacino, J S.]]

[[Category: Hurley, J.H.]]

[[Category: Hurley, J H.]]

[[Category: Kato, Y.]]

[[Category: Misra, S.]]

Line 25:

Line 24:

[[Category: vhs domain]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov 12 17~~:59:~~36 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:44:26 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1lf8.gif|left|200px]]<br />
+[[Image:1lf8.gif|left|200px]]<br /><applet load="1lf8" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1lf8" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1lf8, resolution 2.30&Aring;" />
 '''Complex of GGA3-VHS Domain and CI-MPR C-terminal Phosphopeptide'''<br />
 ==Overview==
-Phosphorylation of the cytosolic tails of transmembrane receptors can, regulate their intracellular trafficking. The structural basis for such, regulation, however, has not been explained in most cases. The cytosolic, tail of the cation-independent mannose 6-phosphate receptor contains a, serine residue within an acidic-cluster dileucine signal that is important, for the function of the receptor in the biosynthetic sorting of lysosomal, hydrolases. We show here that phosphorylation of this Ser enhances, interactions of the signal with its recognition module, the VHS domain of, the GGA proteins. Crystallographic analyses demonstrate that the, phosphoserine residue interacts electrostatically with two basic residues, on the VHS domain of GGA3, thus providing an additional point of, attachment of the acidic-cluster dileucine signal to its recognition, module.
+Phosphorylation of the cytosolic tails of transmembrane receptors can regulate their intracellular trafficking. The structural basis for such regulation, however, has not been explained in most cases. The cytosolic tail of the cation-independent mannose 6-phosphate receptor contains a serine residue within an acidic-cluster dileucine signal that is important for the function of the receptor in the biosynthetic sorting of lysosomal hydrolases. We show here that phosphorylation of this Ser enhances interactions of the signal with its recognition module, the VHS domain of the GGA proteins. Crystallographic analyses demonstrate that the phosphoserine residue interacts electrostatically with two basic residues on the VHS domain of GGA3, thus providing an additional point of attachment of the acidic-cluster dileucine signal to its recognition module.
 ==Disease==
@@ Line 11: / Line 10: @@
 ==About this Structure==
-LF8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LF8 OCA].
+LF8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LF8 OCA].
 ==Reference==
@@ Line 17: / Line 16: @@
 [[Category: Homo sapiens]]
 [[Category: Protein complex]]
-[[Category: Bonifacino, J.S.]]
+[[Category: Bonifacino, J S.]]
-[[Category: Hurley, J.H.]]
+[[Category: Hurley, J H.]]
 [[Category: Kato, Y.]]
 [[Category: Misra, S.]]
@@ Line 25: / Line 24: @@
 [[Category: vhs domain]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:59:36 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:44:26 2008''