1ljx: Difference between revisions

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[[Image:1ljx.gif|left|200px]]<br /><applet load="1ljx" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1ljx.gif|left|200px]]<br /><applet load="1ljx" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1ljx, resolution 1.64Å" />

'''THE STRUCTURE OF D(TPGPCPGPCPA)2 AT 293K: COMPARISON OF THE EFFECT OF SEQUENCE AND TEMPERATURE'''<br />

==Overview==

The crystal structure of a hexameric DNA fragment with the sequence, d(TGCGCA)(2) has been solved and refined at 293 K at a resolution of 1.64, A. The molecule adopts a left-handed Z-type helical conformation which is, common in alternating pyrimidine-purine sequences. The presence of A.T, base pairs at the two terminals does not perturb the structure to any, great degree. However, several sequence-specific microstructural changes, are noticeable. The structure of the identical sequence determined at 120, K involving somewhat different crystallization conditions has been, reported previously [Harper et al. (1998), Acta Cryst. D54, 1273-1284]. A, comparison of the present structure with that at low temperature and with, that of d(CGCGCG)(2) shows that the effect of the change in sequence is, greater than the combined effect of changes in temperature and, environment.

The crystal structure of a hexameric DNA fragment with the sequence d(TGCGCA)(2) has been solved and refined at 293 K at a resolution of 1.64 A. The molecule adopts a left-handed Z-type helical conformation which is common in alternating pyrimidine-purine sequences. The presence of A.T base pairs at the two terminals does not perturb the structure to any great degree. However, several sequence-specific microstructural changes are noticeable. The structure of the identical sequence determined at 120 K involving somewhat different crystallization conditions has been reported previously [Harper et al. (1998), Acta Cryst. D54, 1273-1284]. A comparison of the present structure with that at low temperature and with that of d(CGCGCG)(2) shows that the effect of the change in sequence is greater than the combined effect of changes in temperature and environment.

==About this Structure==

1LJX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1LJX OCA].

1LJX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LJX OCA].

==Reference==

Line 13:

[[Category: Protein complex]]

[[Category: Gautham, N.]]

[[Category: Kumar, P.Satheesh.]]

[[Category: Kumar, P Satheesh.]]

[[Category: Rajan, S.S.]]

[[Category: Rajan, S S.]]

[[Category: Thiyagarajan, S.]]

[[Category: MG]]

[[Category: z-dna hexamer with at base pairs at the terminal]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sun Nov 25 03~~:03:~~13 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:45:39 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1ljx.gif|left|200px]]<br /><applet load="1ljx" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ljx.gif|left|200px]]<br /><applet load="1ljx" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1ljx, resolution 1.64&Aring;" />
 '''THE STRUCTURE OF D(TPGPCPGPCPA)2 AT 293K: COMPARISON OF THE EFFECT OF SEQUENCE AND TEMPERATURE'''<br />
 ==Overview==
-The crystal structure of a hexameric DNA fragment with the sequence, d(TGCGCA)(2) has been solved and refined at 293 K at a resolution of 1.64, A. The molecule adopts a left-handed Z-type helical conformation which is, common in alternating pyrimidine-purine sequences. The presence of A.T, base pairs at the two terminals does not perturb the structure to any, great degree. However, several sequence-specific microstructural changes, are noticeable. The structure of the identical sequence determined at 120, K involving somewhat different crystallization conditions has been, reported previously [Harper et al. (1998), Acta Cryst. D54, 1273-1284]. A, comparison of the present structure with that at low temperature and with, that of d(CGCGCG)(2) shows that the effect of the change in sequence is, greater than the combined effect of changes in temperature and, environment.
+The crystal structure of a hexameric DNA fragment with the sequence d(TGCGCA)(2) has been solved and refined at 293 K at a resolution of 1.64 A. The molecule adopts a left-handed Z-type helical conformation which is common in alternating pyrimidine-purine sequences. The presence of A.T base pairs at the two terminals does not perturb the structure to any great degree. However, several sequence-specific microstructural changes are noticeable. The structure of the identical sequence determined at 120 K involving somewhat different crystallization conditions has been reported previously [Harper et al. (1998), Acta Cryst. D54, 1273-1284]. A comparison of the present structure with that at low temperature and with that of d(CGCGCG)(2) shows that the effect of the change in sequence is greater than the combined effect of changes in temperature and environment.
 ==About this Structure==
-LJX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LJX OCA].
+LJX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LJX OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Protein complex]]
 [[Category: Gautham, N.]]
-[[Category: Kumar, P.Satheesh.]]
+[[Category: Kumar, P Satheesh.]]
-[[Category: Rajan, S.S.]]
+[[Category: Rajan, S S.]]
 [[Category: Thiyagarajan, S.]]
 [[Category: MG]]
 [[Category: z-dna hexamer with at base pairs at the terminal]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:03:13 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:45:39 2008''