1lk2: Difference between revisions

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[[Image:1lk2.gif|left|200px]]<br /><applet load="1lk2" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1lk2.gif|left|200px]]<br /><applet load="1lk2" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1lk2, resolution 1.35Å" />

'''1.35A crystal structure of H-2Kb complexed with the GNYSFYAL peptide'''<br />

==Overview==

We identify and consider some characteristics of a peptide antagonist for, the Ag-specific receptor on 2C cells (the 2C TCR). The peptide, GNYSFYAL, (called GNY), binds to H-2K(b), and a very high-resolution crystal, structure of the GNY-K(b) complex at 1.35 A is described. Although the GNY, peptide does not bind to L(d), the potency of GNY-K(b) as an antagonist is, evident from its ability to specifically inhibit 2C TCR-mediated reactions, to an allogenic agonist complex (QLSPFPFDL-L(d)), as well as to a, syngeneic agonist complex (SIYRYYGL-K(b)). The crystal structure and the, activities of alanine-substituted peptide variants point to the properties, of the peptide P4 side chain and the conformation of the Tyr-P6 side chain, as the structural determinants of GNYSFYAL antagonist activity.

We identify and consider some characteristics of a peptide antagonist for the Ag-specific receptor on 2C cells (the 2C TCR). The peptide, GNYSFYAL (called GNY), binds to H-2K(b), and a very high-resolution crystal structure of the GNY-K(b) complex at 1.35 A is described. Although the GNY peptide does not bind to L(d), the potency of GNY-K(b) as an antagonist is evident from its ability to specifically inhibit 2C TCR-mediated reactions to an allogenic agonist complex (QLSPFPFDL-L(d)), as well as to a syngeneic agonist complex (SIYRYYGL-K(b)). The crystal structure and the activities of alanine-substituted peptide variants point to the properties of the peptide P4 side chain and the conformation of the Tyr-P6 side chain as the structural determinants of GNYSFYAL antagonist activity.

==About this Structure==

1LK2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with NAG, PO4, MRD and MPD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1LK2 OCA].

1LK2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=MRD:'>MRD</scene> and <scene name='pdbligand=MPD:'>MPD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LK2 OCA].

==Reference==

Line 14:

[[Category: Protein complex]]

[[Category: Eisen, H.]]

[[Category: Luz, J.G.]]

[[Category: Luz, J G.]]

[[Category: Rudolph, M.G.]]

[[Category: Rudolph, M G.]]

[[Category: Wilson, I.A.]]

[[Category: Wilson, I A.]]

[[Category: MPD]]

[[Category: MRD]]

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[[Category: high resolution]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 20~~:36:~~58 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:45:41 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1lk2.gif|left|200px]]<br /><applet load="1lk2" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1lk2.gif|left|200px]]<br /><applet load="1lk2" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1lk2, resolution 1.35&Aring;" />
 '''1.35A crystal structure of H-2Kb complexed with the GNYSFYAL peptide'''<br />
 ==Overview==
-We identify and consider some characteristics of a peptide antagonist for, the Ag-specific receptor on 2C cells (the 2C TCR). The peptide, GNYSFYAL, (called GNY), binds to H-2K(b), and a very high-resolution crystal, structure of the GNY-K(b) complex at 1.35 A is described. Although the GNY, peptide does not bind to L(d), the potency of GNY-K(b) as an antagonist is, evident from its ability to specifically inhibit 2C TCR-mediated reactions, to an allogenic agonist complex (QLSPFPFDL-L(d)), as well as to a, syngeneic agonist complex (SIYRYYGL-K(b)). The crystal structure and the, activities of alanine-substituted peptide variants point to the properties, of the peptide P4 side chain and the conformation of the Tyr-P6 side chain, as the structural determinants of GNYSFYAL antagonist activity.
+We identify and consider some characteristics of a peptide antagonist for the Ag-specific receptor on 2C cells (the 2C TCR). The peptide, GNYSFYAL (called GNY), binds to H-2K(b), and a very high-resolution crystal structure of the GNY-K(b) complex at 1.35 A is described. Although the GNY peptide does not bind to L(d), the potency of GNY-K(b) as an antagonist is evident from its ability to specifically inhibit 2C TCR-mediated reactions to an allogenic agonist complex (QLSPFPFDL-L(d)), as well as to a syngeneic agonist complex (SIYRYYGL-K(b)). The crystal structure and the activities of alanine-substituted peptide variants point to the properties of the peptide P4 side chain and the conformation of the Tyr-P6 side chain as the structural determinants of GNYSFYAL antagonist activity.
 ==About this Structure==
-LK2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with NAG, PO4, MRD and MPD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LK2 OCA].
+LK2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=MRD:'>MRD</scene> and <scene name='pdbligand=MPD:'>MPD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LK2 OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Protein complex]]
 [[Category: Eisen, H.]]
-[[Category: Luz, J.G.]]
+[[Category: Luz, J G.]]
-[[Category: Rudolph, M.G.]]
+[[Category: Rudolph, M G.]]
-[[Category: Wilson, I.A.]]
+[[Category: Wilson, I A.]]
 [[Category: MPD]]
 [[Category: MRD]]
@@ Line 25: / Line 25: @@
 [[Category: high resolution]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:36:58 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:45:41 2008''