1lo5: Difference between revisions

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[[Image:1lo5.gif|left|200px]]<br />

[[Image:1lo5.gif|left|200px]]<br /><applet load="1lo5" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1lo5" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1lo5, resolution 3.20Å" />

'''Crystal structure of the D227A variant of Staphylococcal enterotoxin A in complex with human MHC class II'''<br />

==Overview==

Although the biological properties of staphylococcal enterotoxin A (SEA), have been well characterized, structural insights into the interaction, between SEA and major histocompatibilty complex (MHC) class II have only, been obtained by modeling. Here, the crystal structure of the D227A, variant of SEA in complex with human MHC class II has been determined by, X-ray crystallography. SEA(D227A) exclusively binds with its N-terminal, domain to the alpha chain of HLA-DR1. The ability of one SEA molecule to, crosslink two MHC molecules was modeled. It shows that this SEA molecule, cannot interact with the T cell receptor (TCR) while a second SEA molecule, interacts with MHC. Because of its relatively low toxicity, the D227A, variant of SEA is used in tumor therapy.

Although the biological properties of staphylococcal enterotoxin A (SEA) have been well characterized, structural insights into the interaction between SEA and major histocompatibilty complex (MHC) class II have only been obtained by modeling. Here, the crystal structure of the D227A variant of SEA in complex with human MHC class II has been determined by X-ray crystallography. SEA(D227A) exclusively binds with its N-terminal domain to the alpha chain of HLA-DR1. The ability of one SEA molecule to crosslink two MHC molecules was modeled. It shows that this SEA molecule cannot interact with the T cell receptor (TCR) while a second SEA molecule interacts with MHC. Because of its relatively low toxicity, the D227A variant of SEA is used in tumor therapy.

==About this Structure==

1LO5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1LO5 OCA].

1LO5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LO5 OCA].

==Reference==

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[[Category: protein-protein complex]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov 12 18~~:02:~~49 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:46:43 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1lo5.gif|left|200px]]<br />
+[[Image:1lo5.gif|left|200px]]<br /><applet load="1lo5" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1lo5" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1lo5, resolution 3.20&Aring;" />
 '''Crystal structure of the D227A variant of Staphylococcal enterotoxin A in complex with human MHC class II'''<br />
 ==Overview==
-Although the biological properties of staphylococcal enterotoxin A (SEA), have been well characterized, structural insights into the interaction, between SEA and major histocompatibilty complex (MHC) class II have only, been obtained by modeling. Here, the crystal structure of the D227A, variant of SEA in complex with human MHC class II has been determined by, X-ray crystallography. SEA(D227A) exclusively binds with its N-terminal, domain to the alpha chain of HLA-DR1. The ability of one SEA molecule to, crosslink two MHC molecules was modeled. It shows that this SEA molecule, cannot interact with the T cell receptor (TCR) while a second SEA molecule, interacts with MHC. Because of its relatively low toxicity, the D227A, variant of SEA is used in tumor therapy.
+Although the biological properties of staphylococcal enterotoxin A (SEA) have been well characterized, structural insights into the interaction between SEA and major histocompatibilty complex (MHC) class II have only been obtained by modeling. Here, the crystal structure of the D227A variant of SEA in complex with human MHC class II has been determined by X-ray crystallography. SEA(D227A) exclusively binds with its N-terminal domain to the alpha chain of HLA-DR1. The ability of one SEA molecule to crosslink two MHC molecules was modeled. It shows that this SEA molecule cannot interact with the T cell receptor (TCR) while a second SEA molecule interacts with MHC. Because of its relatively low toxicity, the D227A variant of SEA is used in tumor therapy.
 ==About this Structure==
-LO5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LO5 OCA].
+LO5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LO5 OCA].
 ==Reference==
@@ Line 21: / Line 20: @@
 [[Category: protein-protein complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:02:49 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:46:43 2008''