1lti: Difference between revisions

Line 1:

[[Image:1lti.jpg|left|200px]]<br /><applet load="1lti" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1lti.jpg|left|200px]]<br /><applet load="1lti" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1lti, resolution 2.13Å" />

'''HEAT-LABILE ENTEROTOXIN (LT-I) COMPLEX WITH T-ANTIGEN'''<br />

==Overview==

Heat-labile enterotoxin (LT) is part of the cholera toxin (CT) family and, consists of a catalytic A subunit and a B pentamer that serves to, recognize the oligosaccharide part of the GM1 ganglioside receptor. We, report here the crystal structure of heat-labile enterotoxin in complex, with the disaccharide portion of the Thomsen-Friedenreich (T-antigen), tumor marker. The toxin:carbohydrate complex is determined to 2.13 A, resolution, yielding an R-factor of 18.5%. The T-antigen disaccharide, D-Gal-beta 1,3-GalNAc-Ser/Thr, is present in more than 85% of human, carcinomas and monitoring its autoimmune response is used for the early, detection of tumors. Insight into the molecular recognition of this tumor, antigen by sugar binding proteins can benefit the development of a, diagnostic tool for human carcinomas as well as a T-antigen directed, anticancer drug delivery system.

Heat-labile enterotoxin (LT) is part of the cholera toxin (CT) family and consists of a catalytic A subunit and a B pentamer that serves to recognize the oligosaccharide part of the GM1 ganglioside receptor. We report here the crystal structure of heat-labile enterotoxin in complex with the disaccharide portion of the Thomsen-Friedenreich (T-antigen) tumor marker. The toxin:carbohydrate complex is determined to 2.13 A resolution, yielding an R-factor of 18.5%. The T-antigen disaccharide, D-Gal-beta 1,3-GalNAc-Ser/Thr, is present in more than 85% of human carcinomas and monitoring its autoimmune response is used for the early detection of tumors. Insight into the molecular recognition of this tumor antigen by sugar binding proteins can benefit the development of a diagnostic tool for human carcinomas as well as a T-antigen directed anticancer drug delivery system.

==About this Structure==

1LTI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with GAL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1LTI OCA].

1LTI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=GAL:'>GAL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LTI OCA].

==Reference==

Line 13:

[[Category: Escherichia coli]]

[[Category: Protein complex]]

[[Category: Akker, F.Van.Den.]]

[[Category: Akker, F Van Den.]]

[[Category: Hol, W.G.J.]]

[[Category: Hol, W G.J.]]

[[Category: GAL]]

[[Category: adp-ribosyl transferase]]

[[Category: enterotoxin]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 20~~:52:~~12 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:19 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1lti.jpg|left|200px]]<br /><applet load="1lti" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1lti.jpg|left|200px]]<br /><applet load="1lti" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1lti, resolution 2.13&Aring;" />
 '''HEAT-LABILE ENTEROTOXIN (LT-I) COMPLEX WITH T-ANTIGEN'''<br />
 ==Overview==
-Heat-labile enterotoxin (LT) is part of the cholera toxin (CT) family and, consists of a catalytic A subunit and a B pentamer that serves to, recognize the oligosaccharide part of the GM1 ganglioside receptor. We, report here the crystal structure of heat-labile enterotoxin in complex, with the disaccharide portion of the Thomsen-Friedenreich (T-antigen), tumor marker. The toxin:carbohydrate complex is determined to 2.13 A, resolution, yielding an R-factor of 18.5%. The T-antigen disaccharide, D-Gal-beta 1,3-GalNAc-Ser/Thr, is present in more than 85% of human, carcinomas and monitoring its autoimmune response is used for the early, detection of tumors. Insight into the molecular recognition of this tumor, antigen by sugar binding proteins can benefit the development of a, diagnostic tool for human carcinomas as well as a T-antigen directed, anticancer drug delivery system.
+Heat-labile enterotoxin (LT) is part of the cholera toxin (CT) family and consists of a catalytic A subunit and a B pentamer that serves to recognize the oligosaccharide part of the GM1 ganglioside receptor. We report here the crystal structure of heat-labile enterotoxin in complex with the disaccharide portion of the Thomsen-Friedenreich (T-antigen) tumor marker. The toxin:carbohydrate complex is determined to 2.13 A resolution, yielding an R-factor of 18.5%. The T-antigen disaccharide, D-Gal-beta 1,3-GalNAc-Ser/Thr, is present in more than 85% of human carcinomas and monitoring its autoimmune response is used for the early detection of tumors. Insight into the molecular recognition of this tumor antigen by sugar binding proteins can benefit the development of a diagnostic tool for human carcinomas as well as a T-antigen directed anticancer drug delivery system.
 ==About this Structure==
-LTI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with GAL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LTI OCA].
+LTI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=GAL:'>GAL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LTI OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Escherichia coli]]
 [[Category: Protein complex]]
-[[Category: Akker, F.Van.Den.]]
+[[Category: Akker, F Van Den.]]
-[[Category: Hol, W.G.J.]]
+[[Category: Hol, W G.J.]]
 [[Category: GAL]]
 [[Category: adp-ribosyl transferase]]
 [[Category: enterotoxin]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:52:12 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:19 2008''