1lt8: Difference between revisions

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==Overview==

Betaine-homocysteine methyl transferase (BHMT) catalyzes the synthesis of, methionine from betaine and homocysteine (Hcy), utilizing a zinc ion to, activate Hcy. BHMT is a key liver enzyme that is important for, homocysteine homeostasis. X-ray structures of human BHMT in its oxidized, (Zn-free) and reduced (Zn-replete) forms, the latter in complex with the, bisubstrate analog, S(delta-carboxybutyl)-L-homocysteine, were determined, at resolutions of 2.15 A and 2.05 A. BHMT is a (beta/alpha)(8) barrel that, is distorted to construct the substrate and metal binding sites. The zinc, binding sequences G-V/L-N-C and G-G-C-C are at the C termini of strands, beta6 and beta8. Oxidation to the Cys217-Cys299 disulfide and expulsion of, Zn are accompanied by local rearrangements. The structures identify Hcy, binding fingerprints and provide a prototype for the homocysteine, S-methyltransferase family.

Betaine-homocysteine methyl transferase (BHMT) catalyzes the synthesis of methionine from betaine and homocysteine (Hcy), utilizing a zinc ion to activate Hcy. BHMT is a key liver enzyme that is important for homocysteine homeostasis. X-ray structures of human BHMT in its oxidized (Zn-free) and reduced (Zn-replete) forms, the latter in complex with the bisubstrate analog, S(delta-carboxybutyl)-L-homocysteine, were determined at resolutions of 2.15 A and 2.05 A. BHMT is a (beta/alpha)(8) barrel that is distorted to construct the substrate and metal binding sites. The zinc binding sequences G-V/L-N-C and G-G-C-C are at the C termini of strands beta6 and beta8. Oxidation to the Cys217-Cys299 disulfide and expulsion of Zn are accompanied by local rearrangements. The structures identify Hcy binding fingerprints and provide a prototype for the homocysteine S-methyltransferase family.

==About this Structure==

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[[Category: Single protein]]

[[Category: Castro, C.]]

[[Category: Evans, J.C.]]

[[Category: Evans, J C.]]

[[Category: Garrow, T.A.]]

[[Category: Garrow, T A.]]

[[Category: Huddler, D.P.]]

[[Category: Huddler, D P.]]

[[Category: Jiracek, J.]]

[[Category: Ludwig, M.L.]]

[[Category: Ludwig, M L.]]

[[Category: Millian, N.S.]]

[[Category: Millian, N S.]]

[[Category: CBH]]

[[Category: CIT]]

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[[Category: zinc]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-Betaine-homocysteine methyl transferase (BHMT) catalyzes the synthesis of, methionine from betaine and homocysteine (Hcy), utilizing a zinc ion to, activate Hcy. BHMT is a key liver enzyme that is important for, homocysteine homeostasis. X-ray structures of human BHMT in its oxidized, (Zn-free) and reduced (Zn-replete) forms, the latter in complex with the, bisubstrate analog, S(delta-carboxybutyl)-L-homocysteine, were determined, at resolutions of 2.15 A and 2.05 A. BHMT is a (beta/alpha)(8) barrel that, is distorted to construct the substrate and metal binding sites. The zinc, binding sequences G-V/L-N-C and G-G-C-C are at the C termini of strands, beta6 and beta8. Oxidation to the Cys217-Cys299 disulfide and expulsion of, Zn are accompanied by local rearrangements. The structures identify Hcy, binding fingerprints and provide a prototype for the homocysteine, S-methyltransferase family.
+Betaine-homocysteine methyl transferase (BHMT) catalyzes the synthesis of methionine from betaine and homocysteine (Hcy), utilizing a zinc ion to activate Hcy. BHMT is a key liver enzyme that is important for homocysteine homeostasis. X-ray structures of human BHMT in its oxidized (Zn-free) and reduced (Zn-replete) forms, the latter in complex with the bisubstrate analog, S(delta-carboxybutyl)-L-homocysteine, were determined at resolutions of 2.15 A and 2.05 A. BHMT is a (beta/alpha)(8) barrel that is distorted to construct the substrate and metal binding sites. The zinc binding sequences G-V/L-N-C and G-G-C-C are at the C termini of strands beta6 and beta8. Oxidation to the Cys217-Cys299 disulfide and expulsion of Zn are accompanied by local rearrangements. The structures identify Hcy binding fingerprints and provide a prototype for the homocysteine S-methyltransferase family.
 ==About this Structure==
@@ Line 15: / Line 15: @@
 [[Category: Single protein]]
 [[Category: Castro, C.]]
-[[Category: Evans, J.C.]]
+[[Category: Evans, J C.]]
-[[Category: Garrow, T.A.]]
+[[Category: Garrow, T A.]]
-[[Category: Huddler, D.P.]]
+[[Category: Huddler, D P.]]
 [[Category: Jiracek, J.]]
-[[Category: Ludwig, M.L.]]
+[[Category: Ludwig, M L.]]
-[[Category: Millian, N.S.]]
+[[Category: Millian, N S.]]
 [[Category: CBH]]
 [[Category: CIT]]
@@ Line 30: / Line 30: @@
 [[Category: zinc]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:20:14 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:20 2008''