1ltx: Difference between revisions

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[[Image:1ltx.gif|left|200px]]<br /><applet load="1ltx" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1ltx.gif|left|200px]]<br /><applet load="1ltx" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1ltx, resolution 2.70Å" />

'''Structure of Rab Escort Protein-1 in complex with Rab geranylgeranyl transferase and isoprenoid'''<br />

==Overview==

Posttranslational geranylgeranylation of Rab GTPases is catalyzed by Rab, geranylgeranyltransferase (RabGGTase), which consists of a catalytic, alpha/beta heterodimer and an accessory Rab escort protein (REP). The, crystal structure of isoprenoid-bound RabGGTase complexed to REP-1 has, been solved to 2.7 A resolution. The complex interface buries a, surprisingly small surface area of ca. 680 A and is unexpectedly formed by, helices 8, 10, and 12 of the RabGGTase alpha subunit and helices D and E, of REP-1. We demonstrate that the affinity of RabGGTase for REP-1 is, allosterically regulated by phosphoisoprenoid via a long-range, trans-domain signal transduction event. Comparing the structure of REP-1, with the closely related RabGDI, we conclude that the specificity of the, REP:RabGGTase interaction is defined by differently positioned, phenylalanine residues conserved in the REP and GDI subfamilies.

Posttranslational geranylgeranylation of Rab GTPases is catalyzed by Rab geranylgeranyltransferase (RabGGTase), which consists of a catalytic alpha/beta heterodimer and an accessory Rab escort protein (REP). The crystal structure of isoprenoid-bound RabGGTase complexed to REP-1 has been solved to 2.7 A resolution. The complex interface buries a surprisingly small surface area of ca. 680 A and is unexpectedly formed by helices 8, 10, and 12 of the RabGGTase alpha subunit and helices D and E of REP-1. We demonstrate that the affinity of RabGGTase for REP-1 is allosterically regulated by phosphoisoprenoid via a long-range trans-domain signal transduction event. Comparing the structure of REP-1 with the closely related RabGDI, we conclude that the specificity of the REP:RabGGTase interaction is defined by differently positioned phenylalanine residues conserved in the REP and GDI subfamilies.

==About this Structure==

1LTX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with ZN, CL and FAR as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1LTX OCA].

1LTX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=FAR:'>FAR</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LTX OCA].

==Reference==

Line 14:

[[Category: Rattus norvegicus]]

[[Category: Alexandrov, K.]]

[[Category: Goody, R.S.]]

[[Category: Goody, R S.]]

[[Category: Niculae, A.]]

[[Category: Pylypenko, O.]]

Line 20:

[[Category: Reents, R.]]

[[Category: Schlichting, I.]]

[[Category: Thoma, N.H.]]

[[Category: Thoma, N H.]]

[[Category: Waldmann, H.]]

[[Category: CL]]

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[[Category: rab prenylation]]

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@@ Line 1: / Line 1: @@
-[[Image:1ltx.gif|left|200px]]<br /><applet load="1ltx" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ltx.gif|left|200px]]<br /><applet load="1ltx" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1ltx, resolution 2.70&Aring;" />
 '''Structure of Rab Escort Protein-1 in complex with Rab geranylgeranyl transferase and isoprenoid'''<br />
 ==Overview==
-Posttranslational geranylgeranylation of Rab GTPases is catalyzed by Rab, geranylgeranyltransferase (RabGGTase), which consists of a catalytic, alpha/beta heterodimer and an accessory Rab escort protein (REP). The, crystal structure of isoprenoid-bound RabGGTase complexed to REP-1 has, been solved to 2.7 A resolution. The complex interface buries a, surprisingly small surface area of ca. 680 A and is unexpectedly formed by, helices 8, 10, and 12 of the RabGGTase alpha subunit and helices D and E, of REP-1. We demonstrate that the affinity of RabGGTase for REP-1 is, allosterically regulated by phosphoisoprenoid via a long-range, trans-domain signal transduction event. Comparing the structure of REP-1, with the closely related RabGDI, we conclude that the specificity of the, REP:RabGGTase interaction is defined by differently positioned, phenylalanine residues conserved in the REP and GDI subfamilies.
+Posttranslational geranylgeranylation of Rab GTPases is catalyzed by Rab geranylgeranyltransferase (RabGGTase), which consists of a catalytic alpha/beta heterodimer and an accessory Rab escort protein (REP). The crystal structure of isoprenoid-bound RabGGTase complexed to REP-1 has been solved to 2.7 A resolution. The complex interface buries a surprisingly small surface area of ca. 680 A and is unexpectedly formed by helices 8, 10, and 12 of the RabGGTase alpha subunit and helices D and E of REP-1. We demonstrate that the affinity of RabGGTase for REP-1 is allosterically regulated by phosphoisoprenoid via a long-range trans-domain signal transduction event. Comparing the structure of REP-1 with the closely related RabGDI, we conclude that the specificity of the REP:RabGGTase interaction is defined by differently positioned phenylalanine residues conserved in the REP and GDI subfamilies.
 ==About this Structure==
-LTX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with ZN, CL and FAR as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LTX OCA].
+LTX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=FAR:'>FAR</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LTX OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Rattus norvegicus]]
 [[Category: Alexandrov, K.]]
-[[Category: Goody, R.S.]]
+[[Category: Goody, R S.]]
 [[Category: Niculae, A.]]
 [[Category: Pylypenko, O.]]
@@ Line 20: / Line 20: @@
 [[Category: Reents, R.]]
 [[Category: Schlichting, I.]]
-[[Category: Thoma, N.H.]]
+[[Category: Thoma, N H.]]
 [[Category: Waldmann, H.]]
 [[Category: CL]]
@@ Line 30: / Line 30: @@
 [[Category: rab prenylation]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:52:54 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:25 2008''