1lwl: Difference between revisions
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New page: left|200px<br /><applet load="1lwl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lwl, resolution 2.2Å" /> '''Crystal Structure of ... |
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[[Image:1lwl.jpg|left|200px]]<br /><applet load="1lwl" size=" | [[Image:1lwl.jpg|left|200px]]<br /><applet load="1lwl" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1lwl, resolution 2.2Å" /> | caption="1lwl, resolution 2.2Å" /> | ||
'''Crystal Structure of Cytochrome P450-cam with a Fluorescent Probe D-8-Ad (Adamantane-1-carboxylic acid-5-dimethylamino-naphthalene-1-sulfonylamino-octyl-amide)'''<br /> | '''Crystal Structure of Cytochrome P450-cam with a Fluorescent Probe D-8-Ad (Adamantane-1-carboxylic acid-5-dimethylamino-naphthalene-1-sulfonylamino-octyl-amide)'''<br /> | ||
==Overview== | |||
We have synthesized two luminescent probes (D-4-Ad and D-8-Ad) that target cytochrome P450cam. D-4-Ad luminescence is quenched by Forster energy transfer upon binding (Kd = 0.83 muM) but is restored when the probe is displaced from the active site by camphor. In contrast, D-8-Ad (Kd approximately 0.02 muM) is not displaced from the enzyme, even in the presence of a large excess of camphor. The 2.2 A resolution crystal structure of the D-8-Ad:P450cam complex reveals extensive hydrophobic contacts between the probe and the enzyme, which result from the conformational flexibility of the B', F, and G helices. Probes with properties similar to those of D-4-Ad potentially could be useful for screening P450 inhibitors. | |||
==About this Structure== | ==About this Structure== | ||
1LWL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with HEM and DSO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Camphor_5-monooxygenase Camphor 5-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.15.1 1.14.15.1] Full crystallographic information is available from [http:// | 1LWL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with <scene name='pdbligand=HEM:'>HEM</scene> and <scene name='pdbligand=DSO:'>DSO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Camphor_5-monooxygenase Camphor 5-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.15.1 1.14.15.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LWL OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Chiu, R.]] | [[Category: Chiu, R.]] | ||
[[Category: Dunn, A | [[Category: Dunn, A R.]] | ||
[[Category: Goodin, D | [[Category: Goodin, D B.]] | ||
[[Category: Gray, H | [[Category: Gray, H B.]] | ||
[[Category: Hays, A | [[Category: Hays, A M.]] | ||
[[Category: Stout, C | [[Category: Stout, C D.]] | ||
[[Category: Winkler, J | [[Category: Winkler, J R.]] | ||
[[Category: DSO]] | [[Category: DSO]] | ||
[[Category: HEM]] | [[Category: HEM]] | ||
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[[Category: substrate-binding]] | [[Category: substrate-binding]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:49:11 2008'' | ||
