1ma8: Difference between revisions

Line 1:

[[Image:1ma8.gif|left|200px]]<br /><applet load="1ma8" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1ma8.gif|left|200px]]<br /><applet load="1ma8" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1ma8, resolution 1.3Å" />

'''A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine'''<br />

==Overview==

Selenium was incorporated into an oligodeoxynucleotide in the form of, 2'-methylseleno-uridine (U(Se)). The X-ray crystal structure of the duplex, left open bracket d(GCGTA)U(Se)d(ACGC) right open bracket (2) was, determined by the multiwavelength anomalous dispersion (MAD) technique and, refined to a resolution of 1.3 A, demonstrating that selenium can, selectively substitute oxygen in DNA and that the resulting compounds are, chemically stable. Since derivatization at the 2'-alpha-position with, selenium does not affect the preference of the sugar for the C3'-endo, conformation, this strategy is suitable for incorporating selenium into, RNA. The availability of selenium-containing nucleic acids for, crystallographic phasing offers an attractive alternative to the commonly, used halogenated pyrimidines.

Selenium was incorporated into an oligodeoxynucleotide in the form of 2'-methylseleno-uridine (U(Se)). The X-ray crystal structure of the duplex left open bracket d(GCGTA)U(Se)d(ACGC) right open bracket (2) was determined by the multiwavelength anomalous dispersion (MAD) technique and refined to a resolution of 1.3 A, demonstrating that selenium can selectively substitute oxygen in DNA and that the resulting compounds are chemically stable. Since derivatization at the 2'-alpha-position with selenium does not affect the preference of the sugar for the C3'-endo conformation, this strategy is suitable for incorporating selenium into RNA. The availability of selenium-containing nucleic acids for crystallographic phasing offers an attractive alternative to the commonly used halogenated pyrimidines.

==About this Structure==

1MA8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1MA8 OCA].

1MA8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MA8 OCA].

==Reference==

Line 19:

[[Category: Tereshko, V.]]

[[Category: Wawrzak, Z.]]

[[Category: Wilds, C.J.]]

[[Category: Wilds, C J.]]

[[Category: 2'-methylseleno-uridine]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sun Nov 25 03~~:59:~~16 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:53:09 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1ma8.gif|left|200px]]<br /><applet load="1ma8" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ma8.gif|left|200px]]<br /><applet load="1ma8" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1ma8, resolution 1.3&Aring;" />
 '''A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine'''<br />
 ==Overview==
-Selenium was incorporated into an oligodeoxynucleotide in the form of, 2'-methylseleno-uridine (U(Se)). The X-ray crystal structure of the duplex, left open bracket d(GCGTA)U(Se)d(ACGC) right open bracket (2) was, determined by the multiwavelength anomalous dispersion (MAD) technique and, refined to a resolution of 1.3 A, demonstrating that selenium can, selectively substitute oxygen in DNA and that the resulting compounds are, chemically stable. Since derivatization at the 2'-alpha-position with, selenium does not affect the preference of the sugar for the C3'-endo, conformation, this strategy is suitable for incorporating selenium into, RNA. The availability of selenium-containing nucleic acids for, crystallographic phasing offers an attractive alternative to the commonly, used halogenated pyrimidines.
+Selenium was incorporated into an oligodeoxynucleotide in the form of 2'-methylseleno-uridine (U(Se)). The X-ray crystal structure of the duplex left open bracket d(GCGTA)U(Se)d(ACGC) right open bracket (2) was determined by the multiwavelength anomalous dispersion (MAD) technique and refined to a resolution of 1.3 A, demonstrating that selenium can selectively substitute oxygen in DNA and that the resulting compounds are chemically stable. Since derivatization at the 2'-alpha-position with selenium does not affect the preference of the sugar for the C3'-endo conformation, this strategy is suitable for incorporating selenium into RNA. The availability of selenium-containing nucleic acids for crystallographic phasing offers an attractive alternative to the commonly used halogenated pyrimidines.
 ==About this Structure==
-MA8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MA8 OCA].
+MA8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MA8 OCA].
 ==Reference==
@@ Line 19: / Line 19: @@
 [[Category: Tereshko, V.]]
 [[Category: Wawrzak, Z.]]
-[[Category: Wilds, C.J.]]
+[[Category: Wilds, C J.]]
 [[Category: 2'-methylseleno-uridine]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:59:16 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:53:09 2008''