1mij: Difference between revisions

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[[Image:1mij.gif|left|200px]]<br /><applet load="1mij" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1mij.gif|left|200px]]<br /><applet load="1mij" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1mij, resolution 2.05Å" />

'''Crystal Structure of the Homeo-prospero Domain of D. melanogaster Prospero'''<br />

==Overview==

The Prospero transcription factor promotes neural differentiation in, Drosophila, and its activity is tightly regulated by modulating its, subcellular localization. Prospero is exported from the nucleus of neural, precursors but imported into the nucleus of daughter cells, which is, necessary for their proper differentiation. Prospero has a highly, divergent putative homeodomain adjacent to a conserved Prospero domain;, both are required for sequence-specific DNA binding. Here we show that the, structure of these two regions consists of a single structural unit (a, homeo-prospero domain), in which the Prospero domain region is in position, to contribute to DNA binding and also to mask a defined nuclear export, signal that is within the putative homeodomain region. We propose that the, homeo-prospero domain coordinately regulates Prospero nuclear localization, and DNA binding specificity.

The Prospero transcription factor promotes neural differentiation in Drosophila, and its activity is tightly regulated by modulating its subcellular localization. Prospero is exported from the nucleus of neural precursors but imported into the nucleus of daughter cells, which is necessary for their proper differentiation. Prospero has a highly divergent putative homeodomain adjacent to a conserved Prospero domain; both are required for sequence-specific DNA binding. Here we show that the structure of these two regions consists of a single structural unit (a homeo-prospero domain), in which the Prospero domain region is in position to contribute to DNA binding and also to mask a defined nuclear export signal that is within the putative homeodomain region. We propose that the homeo-prospero domain coordinately regulates Prospero nuclear localization and DNA binding specificity.

==About this Structure==

1MIJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1MIJ OCA].

1MIJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MIJ OCA].

==Reference==

Line 13:

[[Category: Drosophila melanogaster]]

[[Category: Single protein]]

[[Category: Doe, C.Q.]]

[[Category: Doe, C Q.]]

[[Category: Matthews, B.W.]]

[[Category: Matthews, B W.]]

[[Category: Ryter, J.M.]]

[[Category: Ryter, J M.]]

[[Category: 4-helix bundle]]

[[Category: dna-binding domain]]

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[[Category: prospero domain]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20~~ 21:26:~~16 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:32 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1mij.gif|left|200px]]<br /><applet load="1mij" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1mij.gif|left|200px]]<br /><applet load="1mij" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1mij, resolution 2.05&Aring;" />
 '''Crystal Structure of the Homeo-prospero Domain of D. melanogaster Prospero'''<br />
 ==Overview==
-The Prospero transcription factor promotes neural differentiation in, Drosophila, and its activity is tightly regulated by modulating its, subcellular localization. Prospero is exported from the nucleus of neural, precursors but imported into the nucleus of daughter cells, which is, necessary for their proper differentiation. Prospero has a highly, divergent putative homeodomain adjacent to a conserved Prospero domain;, both are required for sequence-specific DNA binding. Here we show that the, structure of these two regions consists of a single structural unit (a, homeo-prospero domain), in which the Prospero domain region is in position, to contribute to DNA binding and also to mask a defined nuclear export, signal that is within the putative homeodomain region. We propose that the, homeo-prospero domain coordinately regulates Prospero nuclear localization, and DNA binding specificity.
+The Prospero transcription factor promotes neural differentiation in Drosophila, and its activity is tightly regulated by modulating its subcellular localization. Prospero is exported from the nucleus of neural precursors but imported into the nucleus of daughter cells, which is necessary for their proper differentiation. Prospero has a highly divergent putative homeodomain adjacent to a conserved Prospero domain; both are required for sequence-specific DNA binding. Here we show that the structure of these two regions consists of a single structural unit (a homeo-prospero domain), in which the Prospero domain region is in position to contribute to DNA binding and also to mask a defined nuclear export signal that is within the putative homeodomain region. We propose that the homeo-prospero domain coordinately regulates Prospero nuclear localization and DNA binding specificity.
 ==About this Structure==
-MIJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MIJ OCA].
+MIJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MIJ OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Drosophila melanogaster]]
 [[Category: Single protein]]
-[[Category: Doe, C.Q.]]
+[[Category: Doe, C Q.]]
-[[Category: Matthews, B.W.]]
+[[Category: Matthews, B W.]]
-[[Category: Ryter, J.M.]]
+[[Category: Ryter, J M.]]
 [[Category: 4-helix bundle]]
 [[Category: dna-binding domain]]
@@ Line 21: / Line 21: @@
 [[Category: prospero domain]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:26:16 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:32 2008''