1mjc: Difference between revisions

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[[Image:1mjc.jpg|left|200px]]<br /><applet load="1mjc" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1mjc.jpg|left|200px]]<br /><applet load="1mjc" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1mjc, resolution 2.0Å" />

'''CRYSTAL STRUCTURE OF CSPA, THE MAJOR COLD SHOCK PROTEIN OF ESCHERICHIA COLI'''<br />

==Overview==

The major cold shock protein of Escherichia coli, CspA, produced upon a, rapid downshift in growth temperature, is involved in the transcriptional, regulation of at least two genes. The protein shares high homology with, the nucleic acid-binding domain of the Y-box factors, a family of, eukaryotic proteins involved in transcriptional and translational, regulation. The crystal structure of CspA has been determined at 2-A, resolution and refined to R = 0.187. CspA is composed of five antiparallel, beta-strands forming a closed five-stranded beta-barrel. The, three-dimensional structure of CspA is similar to that of the major cold, shock protein of Bacillus subtilis, CspB, which has recently been, determined at 2.45-A resolution. However, in contrast to CspB, no dimer is, formed in the crystal. The surface of CspA is characteristic for a protein, interacting with single-stranded nucleic acids. Due to the high homology, of the bacterial cold shock proteins with the Y-box factors, E. coli CspA, and B. subtilis CspB define a structural framework for the common cold, shock domain.

The major cold shock protein of Escherichia coli, CspA, produced upon a rapid downshift in growth temperature, is involved in the transcriptional regulation of at least two genes. The protein shares high homology with the nucleic acid-binding domain of the Y-box factors, a family of eukaryotic proteins involved in transcriptional and translational regulation. The crystal structure of CspA has been determined at 2-A resolution and refined to R = 0.187. CspA is composed of five antiparallel beta-strands forming a closed five-stranded beta-barrel. The three-dimensional structure of CspA is similar to that of the major cold shock protein of Bacillus subtilis, CspB, which has recently been determined at 2.45-A resolution. However, in contrast to CspB, no dimer is formed in the crystal. The surface of CspA is characteristic for a protein interacting with single-stranded nucleic acids. Due to the high homology of the bacterial cold shock proteins with the Y-box factors, E. coli CspA and B. subtilis CspB define a structural framework for the common cold shock domain.

==About this Structure==

1MJC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1MJC OCA].

1MJC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJC OCA].

==Reference==

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[[Category: transcription regulation]]

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@@ Line 1: / Line 1: @@
-[[Image:1mjc.jpg|left|200px]]<br /><applet load="1mjc" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1mjc.jpg|left|200px]]<br /><applet load="1mjc" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1mjc, resolution 2.0&Aring;" />
 '''CRYSTAL STRUCTURE OF CSPA, THE MAJOR COLD SHOCK PROTEIN OF ESCHERICHIA COLI'''<br />
 ==Overview==
-The major cold shock protein of Escherichia coli, CspA, produced upon a, rapid downshift in growth temperature, is involved in the transcriptional, regulation of at least two genes. The protein shares high homology with, the nucleic acid-binding domain of the Y-box factors, a family of, eukaryotic proteins involved in transcriptional and translational, regulation. The crystal structure of CspA has been determined at 2-A, resolution and refined to R = 0.187. CspA is composed of five antiparallel, beta-strands forming a closed five-stranded beta-barrel. The, three-dimensional structure of CspA is similar to that of the major cold, shock protein of Bacillus subtilis, CspB, which has recently been, determined at 2.45-A resolution. However, in contrast to CspB, no dimer is, formed in the crystal. The surface of CspA is characteristic for a protein, interacting with single-stranded nucleic acids. Due to the high homology, of the bacterial cold shock proteins with the Y-box factors, E. coli CspA, and B. subtilis CspB define a structural framework for the common cold, shock domain.
+The major cold shock protein of Escherichia coli, CspA, produced upon a rapid downshift in growth temperature, is involved in the transcriptional regulation of at least two genes. The protein shares high homology with the nucleic acid-binding domain of the Y-box factors, a family of eukaryotic proteins involved in transcriptional and translational regulation. The crystal structure of CspA has been determined at 2-A resolution and refined to R = 0.187. CspA is composed of five antiparallel beta-strands forming a closed five-stranded beta-barrel. The three-dimensional structure of CspA is similar to that of the major cold shock protein of Bacillus subtilis, CspB, which has recently been determined at 2.45-A resolution. However, in contrast to CspB, no dimer is formed in the crystal. The surface of CspA is characteristic for a protein interacting with single-stranded nucleic acids. Due to the high homology of the bacterial cold shock proteins with the Y-box factors, E. coli CspA and B. subtilis CspB define a structural framework for the common cold shock domain.
 ==About this Structure==
-MJC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MJC OCA].
+MJC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJC OCA].
 ==Reference==
@@ Line 17: / Line 17: @@
 [[Category: transcription regulation]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:27:15 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:55 2008''