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New page: left|200px<br /><applet load="1mnx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mnx" /> '''The Solution Structure of the Loop E Region ...
 
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[[Image:1mnx.gif|left|200px]]<br /><applet load="1mnx" size="450" color="white" frame="true" align="right" spinBox="true"  
[[Image:1mnx.gif|left|200px]]<br /><applet load="1mnx" size="350" color="white" frame="true" align="right" spinBox="true"  
caption="1mnx" />
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'''The Solution Structure of the Loop E Region of the 5S rRNA from Spinach Chloroplasts.'''<br />
'''The Solution Structure of the Loop E Region of the 5S rRNA from Spinach Chloroplasts.'''<br />


==Overview==
==Overview==
A structure has been obtained for the loop E region of the 5S rRNA from, Spinacia oleracia chloroplast ribosomes using residual dipolar coupling, data as well as NOE, J coupling and chemical shift information. Even, though the loop E sequence of this chloroplast 5S rRNA differs from that, of Escherichia coli loop E at approximately 40% of its positions, its, conformation is remarkably similar to that of E.coli loop E. Consistent, with this conclusion, ribosomal protein L25 from E.coli, which binds to, the loop E region of both intact E.coli 5S rRNA and to oligonucleotides, containing that sequence, also binds to the chloroplast-derived, oligonucleotide discussed here.
A structure has been obtained for the loop E region of the 5S rRNA from Spinacia oleracia chloroplast ribosomes using residual dipolar coupling data as well as NOE, J coupling and chemical shift information. Even though the loop E sequence of this chloroplast 5S rRNA differs from that of Escherichia coli loop E at approximately 40% of its positions, its conformation is remarkably similar to that of E.coli loop E. Consistent with this conclusion, ribosomal protein L25 from E.coli, which binds to the loop E region of both intact E.coli 5S rRNA and to oligonucleotides containing that sequence, also binds to the chloroplast-derived oligonucleotide discussed here.


==About this Structure==
==About this Structure==
1MNX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MNX OCA].  
1MNX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MNX OCA].  


==Reference==
==Reference==
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[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Spinacia oleracea]]
[[Category: Spinacia oleracea]]
[[Category: Moore, P.B.]]
[[Category: Moore, P B.]]
[[Category: Vallurupalli, P.]]
[[Category: Vallurupalli, P.]]
[[Category: 5s rrna]]
[[Category: 5s rrna]]
[[Category: loop e]]
[[Category: loop e]]


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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:57:08 2008''

Revision as of 11:57, 21 February 2008

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1mnx

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The Solution Structure of the Loop E Region of the 5S rRNA from Spinach Chloroplasts.

Overview

A structure has been obtained for the loop E region of the 5S rRNA from Spinacia oleracia chloroplast ribosomes using residual dipolar coupling data as well as NOE, J coupling and chemical shift information. Even though the loop E sequence of this chloroplast 5S rRNA differs from that of Escherichia coli loop E at approximately 40% of its positions, its conformation is remarkably similar to that of E.coli loop E. Consistent with this conclusion, ribosomal protein L25 from E.coli, which binds to the loop E region of both intact E.coli 5S rRNA and to oligonucleotides containing that sequence, also binds to the chloroplast-derived oligonucleotide discussed here.

About this Structure

1MNX is a Protein complex structure of sequences from Spinacia oleracea. Full crystallographic information is available from OCA.

Reference

The solution structure of the loop E region of the 5S rRNA from spinach chloroplasts., Vallurupalli P, Moore PB, J Mol Biol. 2003 Jan 31;325(5):843-56. PMID:12527295

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