1myu: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: left|200px<br /><applet load="1myu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1myu" /> '''Lipid induced conformation of the tachykinin... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1myu.gif|left|200px]]<br /><applet load="1myu" size=" | [[Image:1myu.gif|left|200px]]<br /><applet load="1myu" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1myu" /> | caption="1myu" /> | ||
'''Lipid induced conformation of the tachykinin peptide Kassinin'''<br /> | '''Lipid induced conformation of the tachykinin peptide Kassinin'''<br /> | ||
==Overview== | ==Overview== | ||
Both the aqueous and lipid-induced structure of Kassinin, a dodecapeptide | Both the aqueous and lipid-induced structure of Kassinin, a dodecapeptide of amphibian origin, has been studied by two-dimensional proton nuclear magnetic resonance (2D 1H-NMR) spectroscopy and distance geometry calculations. Unambiguous NMR assignments of protons have been made with the aid of correlation spectroscopy (DQF-COSY and TOCSY) experiments and nuclear Overhauser effect spectroscopy (NOESY and ROESY) experiments. The distance constraints obtained from the NMR data have been utilized in a distance geometry algorithm to generate a family of structures, which have been refined using restrained energy minimization and dynamics. These data show that, while in water Kassinin prefers to be in an extended chain conformation, in the presence of perdeuterated dodecylphosphocholine (DPC) micelles, a membrane model system, helical conformation is induced in the central core and C-terminal region (K4-M12) of the peptide. N-terminus though less defined also displays some degree of order and a possible turn structure. The conformation adopted by Kassinin in the presence of DPC micelles is consistent with the structural motif typical of neurokinin-1 selective agonists and with that reported for Eledoisin in hydrophobic environment. | ||
==About this Structure== | ==About this Structure== | ||
1MYU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | 1MYU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MYU OCA]. | ||
==Reference== | ==Reference== | ||
Lipid induced conformation of the tachykinin peptide Kassinin., Grace RC, Lynn AM, Cowsik SM, J Biomol Struct Dyn. 2001 Feb;18(4):611-21, 623-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11245256 11245256] | Lipid induced conformation of the tachykinin peptide Kassinin., Grace RC, Lynn AM, Cowsik SM, J Biomol Struct Dyn. 2001 Feb;18(4):611-21, 623-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11245256 11245256] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Cowsik, S | [[Category: Cowsik, S M.]] | ||
[[Category: Grace, R | [[Category: Grace, R C.]] | ||
[[Category: Lynn, A | [[Category: Lynn, A M.]] | ||
[[Category: 3-10 helix]] | [[Category: 3-10 helix]] | ||
[[Category: dpc micelles]] | [[Category: dpc micelles]] | ||
| Line 20: | Line 20: | ||
[[Category: lipid induced conformation]] | [[Category: lipid induced conformation]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:00:26 2008'' | ||
