1n24: Difference between revisions
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New page: left|200px<br /><applet load="1n24" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n24, resolution 2.30Å" /> '''(+)-Bornyl diphospha... |
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[[Image:1n24.gif|left|200px]]<br /><applet load="1n24" size=" | [[Image:1n24.gif|left|200px]]<br /><applet load="1n24" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1n24, resolution 2.30Å" /> | caption="1n24, resolution 2.30Å" /> | ||
'''(+)-Bornyl diphosphate synthase: Complex with Mg and product'''<br /> | '''(+)-Bornyl diphosphate synthase: Complex with Mg and product'''<br /> | ||
==Overview== | ==Overview== | ||
The x-ray crystal structure of dimeric (+)-bornyl diphosphate synthase, a | The x-ray crystal structure of dimeric (+)-bornyl diphosphate synthase, a metal-requiring monoterpene cyclase from Salvia officinalis, is reported at 2.0-A resolution. Each monomer contains two alpha-helical domains: the C-terminal domain catalyzes the cyclization of geranyl diphosphate, orienting and stabilizing multiple reactive carbocation intermediates; the N-terminal domain has no clearly defined function, although its N terminus caps the active site in the C-terminal domain during catalysis. Structures of complexes with aza analogues of substrate and carbocation intermediates, as well as complexes with pyrophosphate and bornyl diphosphate, provide "snapshots" of the terpene cyclization cascade. | ||
==About this Structure== | ==About this Structure== | ||
1N24 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salvia_officinalis Salvia officinalis] with MG and BP2 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Geranyl-diphosphate_cyclase Geranyl-diphosphate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.8 5.5.1.8] Full crystallographic information is available from [http:// | 1N24 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salvia_officinalis Salvia officinalis] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=BP2:'>BP2</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Geranyl-diphosphate_cyclase Geranyl-diphosphate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.8 5.5.1.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N24 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Salvia officinalis]] | [[Category: Salvia officinalis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Christianson, D | [[Category: Christianson, D W.]] | ||
[[Category: Coates, R | [[Category: Coates, R M.]] | ||
[[Category: Croteau, R | [[Category: Croteau, R B.]] | ||
[[Category: Urbansky, M.]] | [[Category: Urbansky, M.]] | ||
[[Category: Whittington, D | [[Category: Whittington, D A.]] | ||
[[Category: Wise, M | [[Category: Wise, M L.]] | ||
[[Category: BP2]] | [[Category: BP2]] | ||
[[Category: MG]] | [[Category: MG]] | ||
[[Category: terpene synthase fold]] | [[Category: terpene synthase fold]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:01:27 2008'' | ||
