1n4l: Difference between revisions
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New page: left|200px<br /> <applet load="1n4l" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n4l, resolution 2.00Å" /> '''A DNA analogue of t... |
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[[Image:1n4l.gif|left|200px]]<br /> | [[Image:1n4l.gif|left|200px]]<br /><applet load="1n4l" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1n4l" size=" | |||
caption="1n4l, resolution 2.00Å" /> | caption="1n4l, resolution 2.00Å" /> | ||
'''A DNA analogue of the polypurine tract of HIV-1'''<br /> | '''A DNA analogue of the polypurine tract of HIV-1'''<br /> | ||
==Overview== | ==Overview== | ||
The polypurine tract (PPT) from the HIV-1 genome is resistant to digestion | The polypurine tract (PPT) from the HIV-1 genome is resistant to digestion by reverse transcriptase following (-)-strand synthesis and is used to prime (+)-strand synthesis during retroviral replication. We have determined the crystal structure of the asymmetric DNA/DNA analog16-mer duplex (CTTTTTAAAAGAAAAG/CTTTTCTTTTAAAAAG) comprising most of the "visible" portion of the RNA:DNA hybrid from the polypurine tract of HIV-1, which was previously reported in a complex with HIV-1 reverse transcriptase. Our 16-mer completely encompasses a 10-mer DNA duplex analog of the HIV-1 PPT. We report here a detailed analysis of our B' form 16-mer DNA structure, including three full pure A-tracts, as well as a comparative structural analysis with polypurine tract and other A-tract-containing nucleic acid structures. Our analysis reveals that the polypurine tract structures share structural features despite being different nucleic acid forms (i.e. DNA:DNA versus RNA:DNA). In addition, the previously reported A-tract-containing DNA molecules bound to topoisomerase I are remarkably similar to our polypurine tract 16-mer structure. On the basis of our analysis, we suggest that the specific topology of long pure A-tracts is remarkably comparable across a wide array of biological environments. | ||
==About this Structure== | ==About this Structure== | ||
1N4L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Moloney_murine_leukemia_virus Moloney murine leukemia virus]. Active as [http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] Full crystallographic information is available from [http:// | 1N4L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Moloney_murine_leukemia_virus Moloney murine leukemia virus]. Active as [http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4L OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: RNA-directed DNA polymerase]] | [[Category: RNA-directed DNA polymerase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Cote , M | [[Category: Cote , M L.]] | ||
[[Category: Georgiadis, M | [[Category: Georgiadis, M M.]] | ||
[[Category: Pflomm, M.]] | [[Category: Pflomm, M.]] | ||
[[Category: mmlv rt; polypurine tract; hiv-1; asymmetric dna; protein-dna complex]] | [[Category: mmlv rt; polypurine tract; hiv-1; asymmetric dna; protein-dna complex]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:02:17 2008'' | ||
