1n5b: Difference between revisions

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[[Image:1n5b.gif|left|200px]]<br /><applet load="1n5b" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1n5b.gif|left|200px]]<br /><applet load="1n5b" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1n5b, resolution 2.00Å" />

'''Crystal Structure Of The Yersinia enterocolitica Molecular Chaperone Syce'''<br />

==Overview==

The crystal structure of the Yersinia enterocolitica molecular-chaperone, protein SycE, which specifically binds the YopE protein, has been solved, to 2.0 A resolution by molecular replacement. The crystal contains two, SycE dimers per asymmetric unit; a novel feature of this crystal, when, compared with closely related SycE structures, is a well ordered, carboxy-terminal peptide in one protomer of each dimer. The peptide binds, a hydrophobic patch of a neighboring molecule in a manner similar to that, seen in a SycE-YopE chaperone-target complex, suggestive of low-affinity, 'self-binding' through which the carboxy-terminal peptide might suppress, counterproductive interactions with non-target proteins in vivo.

The crystal structure of the Yersinia enterocolitica molecular-chaperone protein SycE, which specifically binds the YopE protein, has been solved to 2.0 A resolution by molecular replacement. The crystal contains two SycE dimers per asymmetric unit; a novel feature of this crystal, when compared with closely related SycE structures, is a well ordered carboxy-terminal peptide in one protomer of each dimer. The peptide binds a hydrophobic patch of a neighboring molecule in a manner similar to that seen in a SycE-YopE chaperone-target complex, suggestive of low-affinity 'self-binding' through which the carboxy-terminal peptide might suppress counterproductive interactions with non-target proteins in vivo.

==About this Structure==

1N5B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_enterocolitica Yersinia enterocolitica]. This structure ~~superseeds~~ the now removed PDB entry 1MD1. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1N5B OCA].

1N5B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_enterocolitica Yersinia enterocolitica]. This structure supersedes the now removed PDB entry 1MD1. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5B OCA].

==Reference==

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[[Category: Single protein]]

[[Category: Yersinia enterocolitica]]

[[Category: McKay, D.B.]]

[[Category: McKay, D B.]]

[[Category: Trame, C.B.]]

[[Category: Trame, C B.]]

[[Category: molecular chaperone]]

[[Category: type iii secretion system]]

[[Category: yersinia enterocolitica]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20~~ 21:58:~~29 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:02:27 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1n5b.gif|left|200px]]<br /><applet load="1n5b" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1n5b.gif|left|200px]]<br /><applet load="1n5b" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1n5b, resolution 2.00&Aring;" />
 '''Crystal Structure Of The Yersinia enterocolitica Molecular Chaperone Syce'''<br />
 ==Overview==
-The crystal structure of the Yersinia enterocolitica molecular-chaperone, protein SycE, which specifically binds the YopE protein, has been solved, to 2.0 A resolution by molecular replacement. The crystal contains two, SycE dimers per asymmetric unit; a novel feature of this crystal, when, compared with closely related SycE structures, is a well ordered, carboxy-terminal peptide in one protomer of each dimer. The peptide binds, a hydrophobic patch of a neighboring molecule in a manner similar to that, seen in a SycE-YopE chaperone-target complex, suggestive of low-affinity, 'self-binding' through which the carboxy-terminal peptide might suppress, counterproductive interactions with non-target proteins in vivo.
+The crystal structure of the Yersinia enterocolitica molecular-chaperone protein SycE, which specifically binds the YopE protein, has been solved to 2.0 A resolution by molecular replacement. The crystal contains two SycE dimers per asymmetric unit; a novel feature of this crystal, when compared with closely related SycE structures, is a well ordered carboxy-terminal peptide in one protomer of each dimer. The peptide binds a hydrophobic patch of a neighboring molecule in a manner similar to that seen in a SycE-YopE chaperone-target complex, suggestive of low-affinity 'self-binding' through which the carboxy-terminal peptide might suppress counterproductive interactions with non-target proteins in vivo.
 ==About this Structure==
-N5B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_enterocolitica Yersinia enterocolitica]. This structure superseeds the now removed PDB entry 1MD1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N5B OCA].
+N5B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_enterocolitica Yersinia enterocolitica]. This structure supersedes the now removed PDB entry 1MD1. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5B OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Single protein]]
 [[Category: Yersinia enterocolitica]]
-[[Category: McKay, D.B.]]
+[[Category: McKay, D B.]]
-[[Category: Trame, C.B.]]
+[[Category: Trame, C B.]]
 [[Category: molecular chaperone]]
 [[Category: type iii secretion system]]
 [[Category: yersinia enterocolitica]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:58:29 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:02:27 2008''