1n5z: Difference between revisions

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[[Image:1n5z.gif|left|200px]]<br /><applet load="1n5z" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1n5z.gif|left|200px]]<br /><applet load="1n5z" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1n5z, resolution 2.70Å" />

'''Complex structure of Pex13p SH3 domain with a peptide of Pex14p'''<br />

==Overview==

While the function of most small signaling domains is confined to binary, ligand interactions, the peroxisomal Pex13p SH3 domain has the unique, capacity of binding to two different ligands, Pex5p and Pex14p. We have, used this domain as a model to decipher its structurally independent, ligand binding sites. By the combined use of X-ray crystallography, NMR, spectroscopy, and circular dichroism, we show that the two ligands bind in, unrelated conformations to patches located at opposite surfaces of this, SH3 domain. Mutations in the Pex13p SH3 domain that abolish interactions, within the Pex13p-Pex5p interface specifically impair PTS1-dependent, protein import into yeast peroxisomes.

While the function of most small signaling domains is confined to binary ligand interactions, the peroxisomal Pex13p SH3 domain has the unique capacity of binding to two different ligands, Pex5p and Pex14p. We have used this domain as a model to decipher its structurally independent ligand binding sites. By the combined use of X-ray crystallography, NMR spectroscopy, and circular dichroism, we show that the two ligands bind in unrelated conformations to patches located at opposite surfaces of this SH3 domain. Mutations in the Pex13p SH3 domain that abolish interactions within the Pex13p-Pex5p interface specifically impair PTS1-dependent protein import into yeast peroxisomes.

==About this Structure==

1N5Z is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1N5Z OCA].

1N5Z is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5Z OCA].

==Reference==

Line 16:

[[Category: Distel, B.]]

[[Category: Douangamath, A.]]

[[Category: Filipp, F.V.]]

[[Category: Filipp, F V.]]

[[Category: Klein, A.T.J.]]

[[Category: Klein, A T.J.]]

[[Category: Mayans, O.M.]]

[[Category: Mayans, O M.]]

[[Category: Sattler, M.]]

[[Category: Vega, M.C.]]

[[Category: Vega, M C.]]

[[Category: Voorn-Brouwer, T.]]

[[Category: Wilmanns, M.]]

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[[Category: sh3 domain]]

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@@ Line 1: / Line 1: @@
-[[Image:1n5z.gif|left|200px]]<br /><applet load="1n5z" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1n5z.gif|left|200px]]<br /><applet load="1n5z" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1n5z, resolution 2.70&Aring;" />
 '''Complex structure of Pex13p SH3 domain with a peptide of Pex14p'''<br />
 ==Overview==
-While the function of most small signaling domains is confined to binary, ligand interactions, the peroxisomal Pex13p SH3 domain has the unique, capacity of binding to two different ligands, Pex5p and Pex14p. We have, used this domain as a model to decipher its structurally independent, ligand binding sites. By the combined use of X-ray crystallography, NMR, spectroscopy, and circular dichroism, we show that the two ligands bind in, unrelated conformations to patches located at opposite surfaces of this, SH3 domain. Mutations in the Pex13p SH3 domain that abolish interactions, within the Pex13p-Pex5p interface specifically impair PTS1-dependent, protein import into yeast peroxisomes.
+While the function of most small signaling domains is confined to binary ligand interactions, the peroxisomal Pex13p SH3 domain has the unique capacity of binding to two different ligands, Pex5p and Pex14p. We have used this domain as a model to decipher its structurally independent ligand binding sites. By the combined use of X-ray crystallography, NMR spectroscopy, and circular dichroism, we show that the two ligands bind in unrelated conformations to patches located at opposite surfaces of this SH3 domain. Mutations in the Pex13p SH3 domain that abolish interactions within the Pex13p-Pex5p interface specifically impair PTS1-dependent protein import into yeast peroxisomes.
 ==About this Structure==
-N5Z is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N5Z OCA].
+N5Z is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5Z OCA].
 ==Reference==
@@ Line 16: / Line 16: @@
 [[Category: Distel, B.]]
 [[Category: Douangamath, A.]]
-[[Category: Filipp, F.V.]]
+[[Category: Filipp, F V.]]
-[[Category: Klein, A.T.J.]]
+[[Category: Klein, A T.J.]]
-[[Category: Mayans, O.M.]]
+[[Category: Mayans, O M.]]
 [[Category: Sattler, M.]]
-[[Category: Vega, M.C.]]
+[[Category: Vega, M C.]]
 [[Category: Voorn-Brouwer, T.]]
 [[Category: Wilmanns, M.]]
@@ Line 27: / Line 27: @@
 [[Category: sh3 domain]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 21:59:23 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:02:34 2008''